Elite event EE-GM3 and methods and kits for identifying such event in biological samples
The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific herbicide tolerance transformation event at a specific location in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
1. A kit comprising a first primer comprising at least 17 contiguous nucleotides of SEQ ID No. 2 from nucleotide 1 to nucleotide 1451 and a second primer comprising at least 17 contiguous nucleotides of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843, or a first primer comprising at least 17 contiguous nucleotides of the complement of SEQ ID No. 3 from nucleotide 241 to nucleotide 1408 and a second primer comprising at least 17 contiguous nucleotides of SEQ ID No. 3 from nucleotide 1 to nucleotide 240.
2. The kit of claim 1 , wherein said first primer comprises a nucleotide sequence of 17 to 200 contiguous nucleotides of SEQ ID No. 2 from nucleotide 1 to nucleotide 1451 and said second primer comprises 17 to 200 contiguous nucleotides of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843, or said first primer comprises a nucleotide sequence of 17 to 200 contiguous nucleotides of the complement of SEQ ID No. 3 from nucleotide 241 to nucleotide 1408 and said second primer comprises 17 to 200 contiguous nucleotides of SEQ ID No. 3 from nucleotide 1 to nucleotide 240.
3. A primer pair comprising a first primer comprising at least 17 contiguous nucleotides of SEQ ID No. 2 from nucleotide 1 to nucleotide 1451 and a second primer comprising at least 17 contiguous nucleotides of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843, or a first primer comprising at least 17 contiguous nucleotides of the complement of SEQ ID No. 3 from nucleotide 241 to nucleotide 1408 and a second primer comprising at least 17 contiguous nucleotides, of SEQ ID No. 3 from nucleotide 1 to nucleotide 240.
4. The primer pair of claim 3 , wherein said first primer comprises a nucleotide sequence of 17 to 200 contiguous nucleotides of SEQ ID No. 2 from nucleotide 1 to nucleotide 1451 and said second primer comprises 17 to 200 contiguous nucleotides of the complement of SEQ ID No. 2 from nucleotide 1452 to nucleotide 1843, or said first primer comprises a nucleotide sequence of 17 to 200 contiguous nucleotides of the complement of SEQ ID No. 3 from nucleotide 241 to nucleotide 1408 and said second primer comprises a nucleotide sequence of 17 to 200 contiguous nucleotides of SEQ ID No. 3 from nucleotide 1 to nucleotide 240.
5. A primer pair comprising a first primer comprising the sequence of SEQ ID No. 5 and a second primer comprising the sequence of SEQ ID No. 4, or comprising a first primer comprising the sequence of SEQ ID No. 5 and a second primer comprising the sequence of SEQ ID No. 7.
6. A kit comprising the primer pair of claim 5 .
7. A primer pair comprising a first primer comprising at least 17 contiguous nucleotides of SEQ ID No. 2 from nucleotide 1 to nucleotide 1451 and a second primer comprising at least 17 contiguous nucleotides of the complement of SEQ ID No. 11 from nucleotide 1452 to nucleotide 16638, or a first primer comprising at least 17 contiguous nucleotides of the complement of SEQ ID No. 3 from nucleotide 241 to nucleotide 1408 and a second primer comprising at least 17 contiguous nucleotides of SEQ ID No. 11 from nucleotide 1452 to nucleotide 16638.
8. A kit comprising the primer pair of claim 7 .