Separation method and separation matrix
The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH 2 ═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.
1. A separation matrix comprising a solid support and copolymer chains bound to said solid support, wherein said copolymer chains comprise units derived from
a) a first monomer of structure CH 2 ═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate group and
b) an N-vinyl amide,
wherein the molar ratio of the units derived from the first monomer to the units derived from the second non-charged monomer is 0.05 to 5.
2. The separation matrix of claim 1 , wherein said first monomer is selected from a group consisting of vinyl sulfonate and allyloxyhydroxypropyl sulfonate.
3. The separation matrix of claim 1 , wherein said first monomer is vinyl sulfonate.
4. The separation matrix of claim 1 , wherein said second non-charged monomer is selected from a group consisting of N-vinyl pyrrolidone, N-vinyl caprolactam, N-vinyl formamide and N-vinyl acetamide.
5. The separation matrix of claim 1 , wherein said second non-charged monomer is N-vinyl pyrrolidone.
6. The separation matrix of claim 1 , wherein in said copolymer chains the molar ratio of the units derived from the first monomer to the units derived from the second non-charged monomer is 0.5 to 2.
7. The separation matrix of claim 1 , having an ion capacity of 20-200 micromol/mL and a pore size corresponding to a KD value of 0.1-0.8, measured with dextran of Mw 110 kDa as the probe molecule.
8. The separation matrix of claim 7 , wherein the ion capacity of said matrix is 30-70 micromol/mL.
9. The separation matrix of claim 1 , wherein the total content of said copolymer chains in the matrix is 2-80 mg/ml.
10. The separation matrix of claim 1 , wherein said solid support comprises a polysaccharide having has a pore size corresponding to a KD value of 0.5-0.9, measured with dextran of Mw 110 kDa as the probe molecule.
11. The separation of claim 1 , wherein said solid support comprises agar or agarose.
12. The separation matrix of claim 11 , wherein the total content of said copolymer chains in the matrix is 2-20 mg/ml.
13. The separation matrix of claim 1 , wherein said solid support comprises crosslinked agarose and wherein said separation matrix has a pore size corresponding to a KD value of 0.2-0.6, measured with dextran of Mw 110 kDa as the probe molecule.
14. A separation matrix comprising a solid support and copolymer chains bound to said solid support, wherein said copolymer chains comprise units derived from
a) a first monomer selected from a group consisting of vinyl sulfonate and allyloxyhydroxypropyl sulfonate and
b) a second non-charged monomer selected from the group consisting of N-vinyl pyrrolidone, N-vinyl caprolactam, N-vinyl formamide and N-vinyl acetamide,
wherein in said copolymer chains the molar ratio of the units derived from the first monomer to the units derived from the second non-charged monomer is 0.5 to 2.
15. The separation matrix of claim 14 , having an ion capacity of 30-70 micromol/mL and a pore size corresponding to a KD value of 0.1-0.8, measured with dextran of Mw 110 kDa as the probe molecule.
16. The separation matrix of claim 14 , wherein the total content of said copolymer chains in the matrix is 2-20 mg/ml.
17. The separation matrix of claim 14 , wherein said solid support agar or agarose having has a pore size corresponding to a KD value of 0.5-0.9, measured with dextran of Mw 110 kDa as the probe molecule.
18. The separation matrix according to claim 14 , wherein said solid support comprises crosslinked agarose and wherein said separation matrix has a pore size corresponding to a KD value of 0.2-0.6, measured with dextran of Mw 110 kDa as the probe molecule.
19. The separation matrix of claim 14 , wherein said solid support comprises crosslinked agarose and wherein said separation matrix has a pore size corresponding to a KD value of 0.2-0.6, measured with dextran of Mw 110 kDa as the probe molecule.
20. A separation matrix comprising a solid support and copolymer chains bound to said solid support having an ion capacity of 30-70 micromol/mL and a pore size corresponding to a KD value of 0.1-0.8, measured with dextran of Mw 110 kDa as the probe molecule, wherein said copolymer chains comprise units derived from
a) vinyl sulfonate and b) N-vinyl pyrrolidone,
wherein in said copolymer chains the molar ratio of the units derived from the first monomer to the units derived from the second non-charged monomer is 0.5 to 2,
wherein the total content of said copolymer chains in the matrix is 2-20 mg/ml, and
wherein said solid support comprises crosslinked agarose and wherein said separation matrix has a pore size corresponding to a KD value of 0.2-0.6, measured with dextran of Mw 110 kDa as the probe molecule.