IP Library › Granted Patent US 10,214,727
Granted Patent B2
US 10,214,727 · App. 15/413,931 · Granted Feb 26, 2019

Platelet-rich plasma compositions and methods of preparation

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Quick Facts
Patent No.
US 10,214,727
App. No.
15/413,931
Granted
Feb 26, 2019
Kind
B2
Abstract

Methods of preparing platelet-rich plasma (PRP) compositions are disclosed which include adding CD34+ cells to the PRP composition. In addition, the concentration of stromal-derived factor-1 (SDF-1) in the PRP composition may be adjusted to a pre-determined value. The compositions may have elevated levels of white blood cells but reduced levels of neutrophils. Compositions produced by the method are also disclosed.

Claims (15)

1. A method of preparing a platelet-rich plasma (PRP) composition comprising:

isolating platelets at a concentration of 151,000/microliter to 7,000,000 per microliter to obtain PRP, and

adding CD34+ cells at a concentration of 1-3×10 9 per liter to 100×10 9 per liter to the PRP to obtain the PRP composition.

2. The method of claim 1 , further comprising the steps of:

determining the concentration of stromal-derived factor in the PRP composition, and

adjusting the concentration of stromal-derived factor-1 (SDF-1) in the PRP composition to a concentration of 100 pg/ml to 5000 pg/ml.

3. The method of claim 2 , wherein the concentration of SDF-1 is adjusted to a concentration of 500 pg/ml to 5000 pg/ml.

4. The method of claim 1 , wherein the platelets are isolated from whole blood.

5. The method of claim 4 , wherein the whole blood is obtained from spleen.

6. The method of claim 1 , wherein the neutrophil level of the PRP is less than 5,000 /μL.

7. The method of claim 6 , wherein the neutrophil level of the PRP is less than 1,000 /μL.

8. The method of claim 1 , wherein the PRP contains a lower concentration of red blood cells or hemoglobin than the concentration in whole blood.

9. The method of claim 1 , wherein the PRP contains a higher concentration of white blood cells than the concentration in whole blood.

10. The method of claim 9 , wherein the white blood cells comprise higher concentrations of lymphocytes and monocytes compared to whole blood and lower concentrations of neutrophils compared to whole blood.

11. The method of claim 1 , wherein the platelets are obtained from spleen or bone marrow.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 6, 2021
From: MISHRA, ALLAN, DR.
To: BLUE ENGINE BIOLOGICS, LLC
Reel/Frame 056759/0943 →