Systems for handling microfluidic droplets
View Patent ↗The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.
1. A method of reducing contamination associated with sample handling, comprising:
providing an aqueous fluid comprising a sample through a sample inlet;
providing an immiscible fluid flowing through a main channel that is in fluidic communication with the sample inlet, wherein the main channel is in a horizontal plane;
partitioning the aqueous fluid with the immiscible fluid to form a plurality of droplets in the main channel, wherein at least one droplet comprises a sample;
flowing the droplets toward a downstream separation chamber that is in fluidic communication with the main channel,
wherein the separation chamber has a wider cross-section than the main channel cross-section and the separation chamber is disposed perpendicular to the main channel; and
separating the plurality of droplets from the immiscible fluid in the separation chamber based on the different densities of the droplets and the immiscible fluid.
2. The method of claim 1 , further comprising collecting the separated droplets.
3. The method of claim 1 , wherein the separated droplets are collected in a vessel.
4. The method of claim 3 , wherein the vessel is a PCR tube or a test tube.
5. The method of claim 3 , after the partitioning step, further comprising introducing one or more reagents to the droplets.
6. The method of claim 5 , wherein the one or more reagents are amplification reagents.
7. The method of claim 6 , wherein the one or more amplification reagents are for a polymerase chain reaction.
8. The method of claim 5 , wherein the one or more reagents are introduced by merging a droplet comprising one or more reagents with a droplet comprising a sample.
9. The method of claim 5 , after the reagent introducing step, further comprising sorting the droplets.
10. The method of claim 1 , wherein the sample comprises a biological material.
11. The method of claim 10 , wherein the biological material is selected from the group consisting of a tissue, cell, particle, protein, antibody, amino acid, nucleotide, small molecule, and pharmaceutical.
12. The method of claim 11 , wherein the biological material further comprises one or more labels.
13. The method of claim 12 , wherein the one or more labels are DNA tags, dyes, quantum dot, or combination thereof.
14. A method of reducing sample loss associated with sample handling, comprising:
providing an aqueous fluid comprising a sample through a sample inlet;
providing an immiscible fluid flowing through a main channel that is in fluidic communication with the sample inlet, wherein the main channel is in a horizontal plane;
partitioning the aqueous fluid with the immiscible fluid to form a plurality of droplets in the main channel;
flowing the droplets toward a downstream separation chamber that is in fluidic communication with the main channel, wherein the separation chamber has a wider cross-section than the main channel cross-section and the separation chamber is disposed perpendicular to the main channel; and
separating the plurality of droplets from the immiscible fluid in the separation chamber based on the different densities of the droplets and the immiscible fluid.
15. The method of claim 14 , after the separation step, further comprising placing the droplets onto a second continuous phase carrier fluid.
16. The method of claim 15 , wherein the second continuous phase carrier fluid comprises a de-stabilizing surfactant.
17. The method of claim 15 , after the placing step, further comprising destabilizing the droplets.