Recombinant adenovirus vectored FMDV vaccines and uses thereof
View Patent ↗The present invention encompasses FMDV vaccines or compositions. The invention encompasses recombinant vectors encoding and expressing FMDV antigens, epitopes or immunogens which can be used to protect animals, in particular ovines, bovines, caprines, or swines, against FMDV.
1. A composition or vaccine comprising a recombinant viral vector that, when expressed, expresses a foot and mouth Disease Virus (FMDV) antigen comprising a polypeptide sequence selected from any one of SEQ ID NOS: 2, 4, 6, and 8.
2. The composition or vaccine of claim 1 , wherein the viral vector is an adenovirus.
3. The composition or vaccine of claim 1 , wherein the viral vector expresses structural and nonstructural FMDV proteins.
4. The composition or vaccine of claim 1 , wherein the viral vector comprises a polynucleotide sequence selected from any one of SEQ ID NOS: 1, 3, 5, and 7.
5. The composition or vaccine of claim 1 , further comprising a pharmaceutically or veterinarily acceptable carrier, excipient, adjuvant, or vehicle.
6. The composition or vaccine of claim 5 , wherein the composition or vaccine is stable out to 3 months when stored at 4° C.
7. The composition or vaccine of claim 5 , wherein the pharmaceutically or veterinarily acceptable carrier, excipient, adjuvant, or vehicle is selected from polyacrylic acid, LF2 emulsion, LR6 emulsion, TS6 emulsion, LR4 emulsion, carbomer, aluminum hydroxide, aluminum phosphate, saponin, CpG, water-in-oil emulsion, oil-in-water emulsion, carbomer-based adjuvant, and adjuvant composition comprising a lipophile, a polymer of acrylic or methacrylic acid, saline, cholesterol, a saponin, and sodium hydroxide.
8. A method of vaccinating an animal susceptible to FMDV infection or eliciting an immune response in the animal against FMDV comprising administering to the animal the vaccine according to claim 1 .
9. A method of vaccinating all animal susceptible to FMDV infection or eliciting an immune response in the animal against FMDV comprising:
administering to the animal a prime-vaccine; and then
administering to the animal a boost-vaccine,
wherein at least one of the prime-vaccine and the boost-vaccine is the vaccine according to claim 1 .
10. The method of claim 9 , wherein the prime-vaccine is the vaccine according to claim 1 , wherein the boost-vaccine comprises an FMDV antigen, a recombinant viral vector that expresses, in vivo, an FMDV antigen, or both, and wherein the method protects the animal from FMDV and/or prevents FMDV disease progression in the animal.
11. The method of claim 9 , wherein the prime-vaccine comprises an FMDV antigen, a recombinant viral vector that expresses, in vivo, an FMDV antigen, or both, wherein the boost-vaccine is the vaccine according to claim 1 , and wherein the method protects the animal from FMDV and/or prevents FMDV disease progression in the animal.
12. The method of claim 8 , wherein the method protects Maternally Derived Antibody-positive (MDA-positive) animals against FMDV infection.