Capillary electrophoresis inkjet dispensing
Devices, systems, and methods of using them are disclosed that position an end of a capillary electrophoresis tube within an internal tapered nozzle region of an inkjet print head or other microfluidic pump. The capillary electrophoresis tube can extend through an inlet of the microfluidic pump and leave space for a sheath liquid to enter the pump and mix with separated analytes eluted from the capillary electrophoresis tube. The small volume of mixed sheath liquid and analyte can then be jetted through the nozzle at a moving surface, either continuously or as discrete droplets. Relative positions on the surface can indicate separation distances of dispensed analytes.
1. A capillary electrophoresis dispensing apparatus, comprising:
a capillary electrophoresis tube having a capillary inlet, a capillary outlet, and a capillary longitudinal axis proximate to the capillary outlet;
a first electrode proximate to and in fluidic connection with the capillary inlet;
a microfluidic pump chamber having an internal region that is configured to be deformed by an impulsive pump element, wherein the capillary electrophoresis tube extends through the internal region of the microfluidic pump chamber;
a microfluidic nozzle having a nozzle outlet, a tapered internal region proximate to the nozzle outlet, and a nozzle longitudinal axis proximate to the nozzle outlet, the microfluidic nozzle in fluidic connection with the microfluidic pump chamber, wherein the capillary outlet of the capillary electrophoresis tube is located within the tapered internal region of the microfluidic nozzle; and
a second electrode in fluidic connection with the capillary outlet.
2. The apparatus of claim 1 , wherein the capillary electrophoresis tube is at least partially filled with a separation buffer or a sieving matrix.
3. The apparatus of claim 1 wherein the capillary electrophoresis tube extends through the microfluidic pump chamber to the tapered internal region of the microfluidic nozzle.
4. The apparatus of claim 1 wherein the capillary longitudinal axis of the capillary electrophoresis tube is coaxial with the nozzle longitudinal axis of the microfluidic nozzle.
5. The apparatus of claim 1 wherein the capillary electrophoresis tube further comprises a capillary electrophoresis tube tapered region proximate to the capillary outlet.
6. The apparatus of claim 1 further comprising:
a non-conducting polymer shell surrounding the microfluidic pump chamber and the impulsive pump element.
7. The apparatus of claim 1 further comprising:
a metal shell surrounding the microfluidic pump chamber and the impulsive pump element, wherein the second electrode is connected with the metal shell.
8. The apparatus of claim 1 further comprising:
a sheath flow tube connected with an impulsive pump inlet, wherein the sheath flow tube is in fluidic connection with a sheath liquid reservoir.
9. The apparatus of claim 8 wherein the second electrode is located within the sheath liquid reservoir.
10. The apparatus of claim 1 wherein the capillary electrophoresis tube is in fluidic connection with a capillary electrophoresis solution reservoir.
11. The apparatus of claim 10 , wherein the first electrode is located within the capillary electrophoresis reservoir.
12. The apparatus of claim 1 further comprising:
an analyte within the capillary electrophoresis tube; or
a sheath liquid within the microfluidic pump chamber.
13. The apparatus of claim 1 wherein the impulsive pump element comprises a piezoelectric material or a thermoresistive material.