Illumination filter system and observation system for a multispectral fluorescence microscope, multispectral fluorescence microscope, and microscopying method
The present invention relates to an illumination filter system ( 2 ) for medical imaging, in particular multispectral fluorescence imaging, as performed e.g. in a microscope ( 1 ) or endoscope, such as a surgical microscope, in particular a surgical multispectral fluorescence microscope, comprising a first optical filter ( 35 ). The present invention also relates to an observation system ( 3 ) for medical imaging, in particular multispectral fluorescence imaging, as performed e.g. in a microscope ( 1 ) or endoscope, in particular a multispectral fluorescence microscope, comprising a beam splitter ( 21 ) adapted to split a light image ( 13 ) into a first light portion ( 16, 17 ) along a first light path ( 18 ) and a second light portion ( 20 ) along a second light path ( 19 ). To improve known illumination filter systems and observation systems, so these systems work with one light source only, are capable of capturing simultaneously at least one fluorescence signal and a signal of visible reflected light and allow a homogeneous illumination for obtaining different images from the object illuminated, the first optical filter ( 36 ) is adapted to quench light of at least one fluorescence excitation band within the visible spectrum in the illumination filter system ( 2 ) of the present invention, and the first light portion ( 16, 17 ) comprises at least one fluorescence emission band (Em.1, Em.2) in the visible spectrum and the second light portion ( 20 ) portion comprises a visible reflected light (VISR) in the observation system ( 3 ) of the present invention.
1. A medical imaging apparatus comprising:
a light source ( 4 );
an observation system ( 3 ) including a beam splitter ( 21 ) adapted to split a light image ( 13 ) into a first light portion ( 16 , 17 ) along a first light path ( 18 ) and a second light portion ( 20 ) along a second light path ( 19 ), wherein the first light portion ( 16 , 17 ) comprises at least one fluorescence emission band (Em.1, Em.2) in the visible spectrum and wherein the second light portion ( 20 ) comprises visible reflected light; and
an illumination filter system ( 2 ) including a first optical filter ( 35 ) adapted to quench light of at least one fluorescence emission band (Em.1, Em.2) within the visible spectrum, wherein the first optical filter is a band-stop filter adapted to transmit all wavelengths of visible light, except for the quenched light of the at least one fluorescence emission band within the visible spectrum;
wherein the first optical filter ( 35 ) is arranged in optical communication with the light source ( 4 ) and/or the observation system ( 3 ).
2. The medical imaging apparatus of claim 1 , wherein the first optical filter ( 35 ) is adapted to quench light with a wavelength of 500-560 nm and/or light with a wavelength of 620-650 nm.
3. The medical imaging apparatus of claim 1 , further comprising a second optical filter ( 36 ) adapted to attenuate light of at least one fluorescence excitation band (Ex.1, Ex.2) by reducing but not quenching the light of at least one fluorescence excitation band (Ex.1, Ex.2).
4. The medical imaging apparatus of claim 3 , wherein the second optical filter ( 36 ) is adapted to attenuate light with a wavelength of 390-420 nm and/or light with a wavelength of 750-800 nm.
5. The medical imaging apparatus of claim 3 , wherein the second optical filter ( 36 ) is configured to be moved from a first operation position ( 37 ), in which the second optical filter ( 36 ) is in optical communication with the first optical filter ( 35 ), to a second operation position ( 38 ), in which the second optical filter ( 36 ) is out of optical communication with the first optical filter ( 35 ).
6. The medical imaging apparatus of claim 3 , wherein the second optical filter ( 36 ) is a band-stop filter adapted to transmit all wavelengths of visible light, except the attenuated fluorescence excitation bands (Ex.1, Ex.2).
7. The medical imaging apparatus of claim 3 , wherein the second optical filter ( 36 ) comprises a spatial band-stop filter pattern ( 43 ), said spatial pattern ( 43 ) having coverage of 100% of a first illumination path ( 47 ) on the second optical filter ( 36 ) and having coverage of less than 100% of a second illumination path ( 48 , 49 , 50 ) on the second optical filter ( 36 ).
8. The medical imaging apparatus of claim 7 , wherein the spatial band-stop filter pattern ( 43 ) extends over a plurality of illumination paths ( 47 - 50 ) provided on the second optical filter ( 36 ) and the coverage is different in each illumination path ( 47 - 50 ).
9. The medical imaging apparatus of claim 1 , wherein the medical imaging apparatus is an endoscope.
10. The medical imaging apparatus of claim 1 , wherein the medical imaging apparatus is a microscope.
11. The medical imaging apparatus of claim 10 , wherein the medical imaging apparatus is a surgical multispectral fluorescence microscope.