Method and composition for biomolecule stabilization
The present invention relates to biomolecule stabilization to provide biomolecules, such as sensitive polymerases, in a convenient ready-to-go format. The invention provides a method and composition in which non-ionic surfactant or detergents of the polyoxyethylene cetyl ether family are used, preferably a Brij reagent or a combination of Brij reagents.
1. A method for stabilizing biomolecules, comprising: treating a biomolecule with a lyophilisation mixture comprising non-ionic surfactant Brij-58 (polyoxyethylene-20-cetyl ether); dispensing said mixture into a receptacle; and freeze-drying said mixture.
2. The method according to claim 1 , wherein the mixture also comprises: a collapse temperature modifier selected from dextran, hydroxyethyl starch, Ficoll and gelatin; a bulking agent selected from a sugar or sugar alcohol, or amino acids; a stabilizing protein; and a buffering agent, preferably Tris HCl.
3. The method according to claim 2 , for stabilization of protein.
4. The method according to claim 3 , for stabilization of nucleic acid polymerase.
5. The method according to claim 4 , wherein said mixture is formed into beads, cakes, conical, flat or square structure depending on the shape of the receptacle into which the composition is dispensed into.
6. A stabilized biomolecule composition, comprising a biomolecule stabilized in a mixture comprising non-ionic surfactant Brij 58 (polyoxyethylene-20-cetyl ether).
7. The biomolecule composition according to claim 6 , stabilized in a mixture comprising 1-20% Ficoll, 5-25% Melezitose, 0.1-5% Brij 58, 0.1-10 mg/mL BSA; and 5-50 mM Tris-HCl pH 9.0.
8. The biomolecule composition according to claim 7 , wherein the stabilized biomolecule is a protein.
9. The biomolecule composition according to claim 8 , wherein the stabilized biomolecule is in freeze dried or liquid format.