Image-aquisition system
An image-acquisition system includes a microscope apparatus that acquires an image of a specimen; a map-image-acquisition portion that controls the microscope apparatus so as to acquire, at a low magnification, a map image including a plurality of anatomical regions in the specimen; an interface portion that allows a user to specify a desired anatomical region as a target region by means of unique IDs assigned to the individual anatomical regions; a processing portion that calculates a spatial position of the target region on the basis of the map image and atlas data having positional information of the individual anatomical regions; and a high-resolution image-acquisition portion that controls, on the basis of the spatial position of the target region, the microscope apparatus so as to acquire a high-resolution image of the target region in the specimen at a magnification that is greater than that of the map image.
1. An image-acquisition system comprising:
an image-acquisition portion that acquires an image of a specimen;
a first image-acquisition control portion that controls the image-acquisition portion so as to acquire, at a first image-capturing magnification, a first image that includes a plurality of study regions in the specimen;
a target-region specifying portion that is operable by a user to specify a desired study region as a target region by means of unique identification information assigned to the individual study regions;
a position-calculating portion that calculates a spatial position of the target region specified by the target-region specifying portion based on the first image acquired by the image-acquisition portion and a reference image having positional information of the individual study regions; and
a second image-acquisition control portion that controls, based on the spatial position of the target region calculated by the position-calculating portion, the image-acquisition portion so as to acquire a second image of the target region in the specimen at a second image-capturing magnification which is a magnification that is greater than the first image-capturing magnification ,
wherein the first image-acquisition control portion controls the image-acquisition portion so as to acquire a plurality of the first images, positions of which are shifted stepwise in an optical-axis direction in the specimen at a first pitch, and
wherein the second image-acquisition control portion controls the image-acquisition portion so as to acquire a plurality of the second images, positions of which are shifted stepwise in the optical-axis direction in the specimen at a second pitch that is finer than the first pitch.
2. An image-acquisition system according to claim 1 , wherein the position-calculating portion performs registration processing for calculating conversion parameters for positioning the reference image with respect to the plurality of first images and calculates the spatial position of the target region based on the calculated conversion parameters.
3. An image-acquisition system according to claim 1 , wherein the target-region specifying portion displays the reference image in which identification information of the target region and boundaries of the individual study regions are indicated.
4. An image-acquisition system according to claim 1 , wherein the first image-acquisition control portion controls the image-acquisition portion so as to acquire an image of the entire specimen as the first image.
5. An image-acquisition system according to claim 1 , wherein the first image-acquisition control portion:
controls the image-acquisition portion so as to acquire images of at least two partial areas of the specimen, a total area of the at least two partial areas being smaller than a total area of the entire specimen, and
combines the acquired images of the at least two partial areas of the specimen with a marked image representing a non-acquired portion of the specimen, to thereby obtain the first image.
6. An image-acquisition system according to claim 5 , wherein the position-calculating portion performs registration processing for calculating conversion parameters for positioning the reference image with respect to the plurality of first images using only the acquired images of the at least two partial areas of the specimen in each respective first image without using the marked image representing the non-acquired portion of the specimen in each respective first image, and calculates the spatial position of the target region based on the calculated conversion parameters.
7. An image-acquisition system comprising:
a microscope apparatus that acquires an image of a specimen; and
a computer that is configured to operate as:
a first image-acquisition control portion that controls the microscope apparatus so as to acquire, at a first image-capturing magnification, a first image that includes a plurality of study regions in the specimen;
a target-region specifying portion that allows a user to specify a desired study region as a target region by means of unique identification information assigned to the individual study regions;
a position-calculating portion that calculates a spatial position of the target region specified by the target-region specifying portion based on the first image acquired by the microscope apparatus and a reference image having positional information of the individual study regions; and
a second image-acquisition control portion that controls, based on the spatial position of the target region calculated by the position-calculating portion, the microscope apparatus so as to acquire a second image of the target region in the specimen at a second image-capturing magnification which is a magnification that is greater than the first image-capturing magnification,
wherein the first image-acquisition control portion controls the microscope apparatus so as to acquire a plurality of the first images, positions of which are shifted stepwise in an optical-axis direction in the specimen at a first pitch, and
wherein the second image-acquisition control portion controls the microscope apparatus so as to acquire a plurality of the second images, positions of which are shifted stepwise in the optical-axis direction in the specimen at a second pitch that is finer than the first pitch.
8. An image-acquisition system according to claim 7 , wherein the position-calculating portion performs registration processing for calculating conversion parameters for positioning the reference image with respect to the plurality of first images and calculates the spatial position of the target region based on the calculated conversion parameters.
9. An image-acquisition system according to claim 7 , wherein the target-region specifying portion displays the reference image in which identification information of the target region and boundaries of the individual study regions are indicated.
10. An image-acquisition system according to claim 7 , wherein the first image-acquisition control portion controls the microscope apparatus so as to acquire an image of the entire specimen as the first image.
11. An image-acquisition system according to claim 7 , wherein the first image-acquisition control portion:
controls the microscope apparatus so as to acquire images of at least two partial areas of the specimen, a total area of the at least two partial areas being smaller than a total area of the entire specimen, and
combines the acquired images of the at least two partial areas of the specimen with a marked image representing a non-acquired portion of the specimen, to thereby obtain the first image.
12. An image-acquisition system according to claim 11 , wherein the position-calculating portion performs registration processing for calculating conversion parameters for positioning the reference image with respect to the plurality of first images using only the acquired images of the at least two partial areas of the specimen in each respective first image without using the marked image representing the non-acquired portion of the specimen in each respective first image, and calculates the spatial position of the target region based on the calculated conversion parameters.