Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof
View Patent ↗The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.
1. A biomass produced from a single isolated strain of thraustochytrid microorganism comprising a triacylglycerol fraction which is at least 60% by weight of the extracted crude oil content of said biomass, wherein from 10% to 30% by weight of the triacylglycerol fraction is eicosapentaenoic acid, wherein said thraustochytrid microorganism is of the genus Schizochytrium or Thraustochytrium , wherein said biomass further comprising an effective amount of at least one added antioxidant to provide oxidative stability.
2. The isolated biomass of claim 1 , wherein the triacylglycerol fraction is between 65% to 95% by weight.
3. The isolated biomass of claim 1 , wherein from 15% to 30% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid.
4. The isolated biomass of claim 1 , wherein at least 25% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid.
5. The isolated biomass of claim 1 , wherein 2% or less by weight of the fatty acids in the triacylglycerol fraction is arachidonic acid.
6. An animal feed for a non-human animal, comprising the biomass of claim 1 .
7. The animal feed of claim 6 , wherein the animal feed is an aquaculture feed.
8. The animal feed of claim 6 , wherein the animal feed is a domestic animal feed, a zoological animal feed, a work animal feed, a livestock feed, or a combination thereof.