Patent
An article for detecting Salmonella microorganisms is provided. The article comprises a highly selective nutrient medium and a plurality of indicator systems. A method of using the article to detect Salmonella microorganisms is also provided.
1. A culture device for detecting a target microorganism, comprising:
a selective growth medium that includes nutrients to facilitate the growth of the target microorganism, Cefsulodin, Nalidixic Acid, Streptomycin, Methicillin, and bile salts;
a first differential indicator system; and
a gelling agent; and
further comprising a nondifferential indicator system to indicate the presence of the target organism.
2. The culture device of claim 1 , wherein the first differential indicator system comprises a pH indicator.
3. The culture device of claim 1 , further comprising a second differential indicator system to indicate the presence of a nontarget microorganism.
4. The culture device of claim 3 , wherein the second differential indicator comprises a chromogenic β-galactosidase enzyme substrate.
5. The culture device of claim 1 , wherein the selective growth medium comprises a dry, rehydratable selective growth medium, wherein the gelling agent comprises a dry, cold water-soluble gelling agent.
6. The culture device of claim 5 , wherein the culture device comprises a thin film culture device.
7. The culture device of claim 1 , wherein the culture device further comprises a third differential indicator system.
8. The culture device of claim 3 , wherein the culture device further comprises a third differential indicator system.
9. A method of using the culture device of claim 1 , comprising contacting the culture device with liquid sample to form an inoculated culture device.
10. The method of claim 9 , further comprising incubating the inoculated culture device for a first period of time.
11. The method of claim 10 , further comprising observing the growth medium for an indication of a possible presence of a target microorganism in the sample.
12. The method of claim 11 , further comprising contacting the growth medium with a second differential indicator system.
13. The method of claim 12 , further comprising contacting the growth medium with a third differential indicator system.
14. The method of claim 13 , further comprising detecting the presence of a target microorganism in the sample, wherein the target microorganism is Salmonella.
15. A method of using the culture device of claim 3 , comprising contacting the culture device with liquid sample to form an inoculated culture device.
16. The method of claim 15 , further comprising incubating the inoculated culture device for a first period of time.
17. The method of claim 16 , further comprising observing the growth medium for an indication of a possible presence of a target microorganism in the sample.
18. The method of claim 17 , further comprising contacting the growth medium with a third differential indicator system.
19. The method of claim 18 , further comprising detecting the presence of a target microorganism in the sample, wherein the target microorganism is Salmonella.
20. The method of claim 15 , further comprising detecting the presence of a target microorganism in the sample, wherein the target microorganism is Salmonella.