Method of reducing titers of antibodies specific for a therapeutic agent
The present invention relates, in general, to a method of treating patients undergoing enzyme replacement therapy (ERT) or other therapy involving the administration of a proteinaceous therapeutic agent as well gene replacement therapy with non-viral or viral vectors, or other therapeutic modality or modalities, used alone or in combination, which involve the administration of exogenous substances for potential therapeutic benefit, including, but not limited to DNA vaccines, siRNA, splice-site switching oligomers (SSOs) as well as RNA-based nanoparticles (RNPs) and nanovaccines. The invention further relates to compounds and compositions suitable for use in such methods.
1. A method of reducing titers of antibodies specific for a therapeutic agent, wherein said therapeutic agent is a proteinaceous replacement for alpha-galactosidase A, in a patient suffering from Fabry disease treated with said therapeutic agent, said method comprising administering to said patient a proteasome inhibitor in an amount and under conditions such that said antibody titers are reduced.
2. A method of reducing the incidence of the formation in a patient suffering from Fabry disease of antibodies specific for a therapeutic agent, wherein said therapeutic agent is a proteinaceous replacement for alpha-galactosidase A, comprising administering to said patient, prior to administration of said therapeutic agent, an amount of a proteasome inhibitor sufficient to effect said reduction.
3. The method according to claim 1 , wherein said therapeutic agent is administered directly to said patient.
4. The method according to claim 2 , wherein said therapeutic agent is administered directly to said patient.
5. The method according to claim 1 , wherein said proteinaceous replacement is recombinant human alpha-galactosidase A.
6. The method according to claim 2 , wherein said proteinaceous replacement is recombinant human alpha-galactosidase A.
7. The method according to claim 1 , wherein said proteasome inhibitor is bortezomib.
8. The method according to claim 2 , wherein said proteasome inhibitor is bortezomib.
9. The method according to claim 1 , wherein said method further comprises administering to said patient a compound that targets or alters antigen presentation or humoral or cell mediated immune response.
10. The method according to claim 2 , wherein said method further comprises administering to said patient a compound that targets or alters antigen presentation or humoral or cell mediated immune response.
11. The method according to claim 1 , wherein said method further comprises administering to said patient a compound that exerts a therapeutic effect against B cells and a compound that targets or alters antigen presentation or humoral or cell mediated immune response.
12. The method according to claim 2 , wherein said method further comprises administering to said patient a compound that exerts a therapeutic effect against B cells and a compound that targets or alters antigen presentation or humoral or cell mediated immune response.
13. The method according to claim 11 , wherein said method comprises administering to said patient rituximab, methotrexate and intravenous gamma globulin.
14. The method according to claim 12 , wherein said method comprises administering to said patient rituximab, methotrexate and intravenous gamma globulin.