MicroRNA compounds and methods for modulating miR-21 activity
Described herein are compositions and methods for the inhibition of miR-21 activity. The compositions have certain nucleoside modification patterns that yield potent inhibitors of miR-21 activity. The compositions may be used to inhibit miR-21, and also to treat diseases associated with abnormal expression of miR-21, such as fibrosis and cancer.
1. A compound comprising a modified oligonucleotide consisting of 11 to 19 linked nucleosides, wherein the nucleobase sequence of the modified oligonucleotide is complementary to miR-21 (SEQ ID NO: 1) and wherein the modified oligonucleotide comprises at least 11 contiguous nucleosides of the following nucleoside pattern V in the 5′ to 3′ orientation:
N M -N B -(N Q -N Q -N B -N B ) 4 -N Z
wherein N M is a modified nucleoside that is not a bicyclic nucleoside;
each N B is a bicyclic nucleoside;
each N Q is a non-bicyclic nucleoside;
N Z is a modified nucleoside; and
wherein each internucleoside linkage is a phosphorothioate linkage.
2. The compound of claim 1 , wherein each bicyclic nucleoside is an S-cEt nucleoside or an LNA nucleoside.
3. The compound of claim 1 , wherein each non-bicyclic nucleoside is independently selected from a β-D-deoxyribonucleoside, a 2′-O-methyl, and a 2′-O-methoxyethyl nucleoside.
4. The compound of claim 1 wherein:
N M is a 2′-O-methoxyethyl nucleoside;
each N B is an S-cEt nucleoside;
each N Q is a β-D-deoxyribonucleoside; and
N Z is a 2′-O-methoxyethyl nucleoside.
5. The compound of claim 1 having the structure:
(SEQ ID NO: 3)
A E C S ATC S A S GTC S U S GAU S A S AGC S U S A E ;
wherein nucleosides not followed by a subscript are β-D-deoxyribonucleosides; nucleosides followed by a subscript “E” are 2′-MOE nucleosides; and nucleosides followed by a subscript “S” are S-cEt nucleosides.
6. The compound of claim 1 , wherein the nucleobase sequence of the modified oligonucleotide is SEQ ID NO: 3, wherein each T is independently selected from T and U.
7. The compound of claim 1 , wherein the modified oligonucleotide has 0 mismatches with respect to the nucleobase sequence of miR-21.
8. The compound of claim 1 , wherein the modified oligonucleotide has 1 or 2 mismatches with respect to the nucleobase sequence of miR-21.
9. A method of inhibiting the activity of miR-21 comprising contacting a cell with a compound of claim 1 .
10. The method of claim 9 , wherein the cell is in vivo or wherein the cell is in vitro.
11. The method of claim 9 wherein the cell is a fibroblast cell, a hyperproliferative cell, a keratinocyte, or a hypoxic cell.
12. A method of decreasing collagen expression in a cell comprising contacting a cell with a compound of claim 1 .
13. A method of treating, preventing or delaying the onset of a disease associated with miR-21 comprising administering to a subject having a disease associated with miR-21 a compound of claim 1 , wherein the disease is fibrosis.
14. The method of claim 13 , wherein the fibrosis is selected from kidney fibrosis, lung fibrosis, liver fibrosis, and cardiac fibrosis.
15. The method of claim 14 , wherein:
a. the kidney fibrosis is present in a subject having a disease selected from glomerulosclerosis, tubulointerstitial fibrosis, IgA nephropathy, interstitial fibrosis/tubular atrophy; chronic kidney damage, glomerular disease, glomerulonephritis, Alport Syndrome, diabetes mellitus, idiopathy focal segmental glomerulosclerosis, membranous nephropathy, collapsing glomerulopathy, chronic recurrent kidney infection, and end stage renal disease;
b. the kidney fibrosis results from acute or repetitive trauma to the kidney;
c. the liver fibrosis is present in a subject having a disease selected from chronic liver injury, hepatitis infection, non-alcoholic steatohepatitis, and cirrhosis;
d. the lung fibrosis is idiopathic pulmonary fibrosis; and/or
e. the subject has chronic obstructive pulmonary disease.
16. The method of claim 13 , wherein the administering improves organ function in the subject, wherein the organ function is selected from cardiac function, pulmonary function, liver function, and kidney function.
17. The method of claim 13 comprising administering at least one therapeutic agent selected from an anti-inflammatory agent, an immunosuppressive agent, an anti-diabetic agent, digoxin, a vasodilator, an angiotensin II converting enzyme (ACE) inhibitors, an angiotensin II receptor blockers (ARB), a calcium channel blocker, an isosorbide dinitrate, a hydralazine, a nitrate, a hydralazine, a beta-blocker, a natriuretic peptides, a heparinoid, and a connective tissue growth factor inhibitor.
18. The method of claim 13 , wherein the subject is a human.
19. The method of claim 13 , wherein the compound is present as a pharmaceutical composition.
20. A pharmaceutical composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier.