Nucleic acid detection cassette
In a nucleic acid detection cassette, a predetermined syringe is crushed to supply a sample solution to an amplification unit via a channel and the amplification unit is heated from outside to amplify sample DNA in the sample solution. The sample solution containing an amplification product is supplied to a detection unit via the channel and a hybridization reaction occurs in the detection unit. Next, another syringe is crushed and an intercalating agent solution is supplied via another predetermined channel. Therefore, a nucleic acid detection cassette capable of automation from amplification of a sample to detection of an electrochemical reaction of the sample is provided.
1. A nucleic acid detection cassette comprising:
a laminated structure including;
an upper plate made of a rigid material,
a packing sheet made of an elastic material, and
a lower plate made of a rigid material, which seals the packing sheet together with the upper plate;
channels including an amplification channel to which a sample solution is supplied, a coupling channel, and a detection channel connected to the amplification channel through the coupling channel in series, which are formed in the laminated structure;
an amplification part formed in the laminated structure, which performs an amplification reaction using a plurality of types of primer sets with sample nucleic acid in the sample solution to produce an amplification product including a target DNA, the amplification part including a plurality of wells and interval channel portions, the wells having inner surfaces on which the primer sets are releasably immobilized, and interval channel portions coupling the wells in series so that the adjacent wells are liquid-jointed by the interval channel portions and the wells and the interval channel portions forms the amplification channel; and
a detection part formed in the laminated structure, and including the detection channel, electrical electrodes arranged in the channel, and nucleic acid probes fixed on the electrical electrodes, wherein the amplification product is supplied from the amplification channel, and the target DNA in the amplification product reacts with the nucleic acid probe so that a hybridize signal is generated from the electrodes to detect a target DNA, wherein
the wells have a width larger than a width of the interval channel portions,
the plurality of types of primer sets which are releasably immobilized on inner walls of the wells for each type, and
a distance between centers of the adjacent wells which is set within 4 mm to 8 mm along an axis of the arrangement of the channel portions, which connects the centers of the adjacent wells, and
the width of the wells is 1.5 to 3.0 times larger than the width of the interval channel portions.
2. The nucleic acid detection cassette of claim 1 , wherein the distance is set within 6 mm to 8 mm.
3. The nucleic acid detection cassette of claim 2 , wherein the width of the wells is 1.7 to 2.3 times larger than the width of the interval channel portions.
4. The nucleic acid detection cassette of claim 1 , wherein the width of the wells is 1.7 to 2.3 times larger than the width of the interval channel portions.
5. The nucleic acid detection cassette of claim 1 , further comprising a first supplying part which supplies the sample solution to the amplification channel, and a second supply part which supplies the amplification product to the detection channel.
6. The nucleic acid detection cassette of claim 1 , wherein the width of the interval channel is in a range from 0.05 to 1.5 mm.
7. The nucleic acid detection cassette of claim 1 , wherein the width of the well is in a range from 0.2 mm to 3.0 mm.