Methods of generating bioactive peptide-bearing antibodies and compositions comprising the same
In one aspect, the invention provides a method of making a bioactive peptide-bearing antibody, or fragment thereof, comprising (a) engrafting the amino acid sequence of at least one bioactive peptide of interest into (i) at least one of CDR-H1, CDR-H2 or CDR-H3 of a heavy chain variable region comprising one or more chicken framework regions and/or (ii) at least one of CDR-L1, CDR-L2 or CDR-L3 of the light chain variable region comprising one or more chicken framework regions, and (b) determining whether the antibody has substantially the same biological activity as the bioactive peptide.
1. A method of inhibiting lectin pathway complement activation in a human subject comprising administering a composition comprising an isolated antibody which comprises a bioactive peptide comprising the amino acid sequence of SEQ ID NO:6, in an amount sufficient to inhibit lectin pathway complement activation in said human subject, wherein said antibody comprises a heavy chain comprising SEQ ID NO:56 and a light chain comprising SEQ ID NO:30.
2. The method of claim 1 , wherein the light chain further comprises a human lambda light chain constant region.