Conversion of biomass to useful intermediates
View Patent ↗An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
1. A non-naturally occurring Pseudomonas not capable of expressing 3-oxoadipate CoA-transferase, and capable of producing 3-oxohexanedioic acid,
wherein the Pseudomonas is capable of growing on at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and
wherein the Pseudomonas lacks genes encoding for PcaI and/or PcaJ.
2. The Pseudomonas of claim 1 , wherein the Pseudomonas lacks the genes encoding for PcaI and PcaJ.
3. The Pseudomonas of claim 1 , wherein the Pseudomonas lacks a gene encoding for PcaI.
4. The Pseudomonas of claim 1 , wherein the Pseudomonas lacks a gene encoding for PcaJ.
5. The Pseudomonas of claim 1 , wherein the Pseudomonas is P. putida KT2440.
6. The Pseudomonas of claim 1 , wherein the cellulose decomposition molecule comprises a sugar molecule.
7. The Pseudomonas of claim 6 , wherein the sugar molecule is at least one of D-xylose or D-glucose.
8. The Pseudomonas of claim 1 , wherein the lignin decomposition molecule comprises an aromatic molecule.
9. The Pseudomonas of claim 8 , wherein the aromatic molecule is at least one of catechol, benzoate, phenol, guaiacol, protecatechuate, ferulate, p-coumarate, vanillate, or 4-hydroxybenzoate.
10. The Pseudomonas of claim 9 , wherein the aromatic molecule is protocatechuate.
11. The Pseudomonas of claim 1 , further comprising an exogenous gene encoding a carboxylase.
12. The Pseudomonas of claim 11 , wherein the exogenous gene encodes for AroY.