Pixelated 2-dimensional fluorescence detection system
View Patent ↗A multiple capillary florescent detection system employing optical fiber bundles that each fiber bundle has more than one fiber illuminating each sample vessel.
1. A fluorescence detection system, comprising:
a plurality of sample vessels comprised of capillaries;
a light source to emit light to excite a fluorescently labelled sample, the system configured to direct light on each sample vessel to illuminate a volume of more than about 100 micrometers×πr2, where r is one-half of the inner diameter of the sample vessel;
a pixelated 2-dimensional detector with horizontal and vertical pixels positioned to detect the fluorescent emissions of the sample, where said more than about 100 micrometers is imaged onto the vertical pixels of said 2-dimensional detector;
where the signal output of said vertical pixels are summed together using an attached computer processor to generate a signal corresponding to said fluorescent emission of the sample.
2. The fluorescence detection system of claim 1 , wherein the light is directed on the sample vessel to illuminate a volume of more than about 500 micrometers×πr2.
3. The fluorescence detection system of claim 1 , wherein the light is directed on the sample vessel to illuminate a volume of more than about 1000 micrometers×πr2.
4. The fluorescence detection system of claim 1 , wherein the light source is an LED.
5. A fluorescence detection system, comprising:
a plurality of sample vessel comprised of capillaries;
a light source to emit light to excite a fluorescently labelled sample, the system configured to direct light on each sample vessel to illuminate a volume of more than about 100 micrometers×πr2, where r is one-half of the inner diameter of the sample vessel;
said light source being optically coupled to an optical fiber bundle that transmits light emitted by the light source to said plurality of sample vessels,
said optical fiber bundle containing at least two individual optical fibers; wherein the optical fiber position from the light entrance and exit are randomized;
a pixelated 2-dimensional detector with horizontal and vertical pixels positioned to detect the fluorescent emissions of the sample, where said more than about 100 micrometers is imaged onto the vertical pixels of said 2-dimensional detector;
where the signal output of said vertical pixels are summed together using an attached computer processor to generate a signal corresponding to said fluorescent emission of the sample.
6. The fluorescence detection system of claim 5 , wherein the light is directed on the sample vessel to illuminate a volume of more than about 500 micrometers×πr2.
7. The fluorescence detection system of claim 5 , wherein the light is directed on the sample vessel to illuminate a volume of more than about 1000 micrometers×πr2.
8. The fluorescence detection system of claim 5 , wherein the light source is an LED.