Anti-CD3 antibodies and methods of use
The invention provides anti-cluster of differentiation 3 (CD3) antibodies and methods of using the same.
1. A bispecific antibody that binds to HER2 and CD3, wherein the bispecific antibody comprises:
(a) an anti-HER2 arm comprising a first binding domain, the first binding domain comprising:
a hypervariable region (HVR)-H1 comprising the amino acid sequence of SEQ ID NO: 169,
an HVR-H2 comprising the amino acid sequence of SEQ ID NO: 170,
an HVR-H3 comprising the amino acid sequence of SEQ ID NO: 171,
an HVR-L1 comprising the amino acid sequence of SEQ ID NO: 172,
an HVR-L2 comprising the amino acid sequence of SEQ ID NO: 173, and
an HVR-L3 comprising the amino acid sequence of SEQ ID NO: 174; and
(b) an anti-CD3 arm comprising a second binding domain, the second binding domain comprising:
an HVR-H1 comprising the amino acid sequence of SEQ ID NO: 1,
an HVR-H2 comprising the amino acid sequence of SEQ ID NO: 2,
an HVR-H3 comprising the amino acid sequence of SEQ ID NO: 3,
an HVR-L1 comprising the amino acid sequence of SEQ ID NO: 4,
an HVR-L2 comprising the amino acid sequence of SEQ ID NO: 5, and
an HVR-L3 comprising the amino acid sequence of SEQ ID NO: 6.
2. The bispecific antibody of claim 1 , wherein the first binding domain comprises:
(a) a heavy chain variable (VH) domain comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 270; (b) a light chain variable (VL) domain comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 271; or (c) a VH domain as in (a) and a VL domain as in (b),
and the second binding domain comprises:
(a) a VH domain comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 184; (b) a VL domain comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 185; or (c) a VH domain as in (a) and a VL domain as in (b).
3. The bispecific antibody of claim 1 , wherein the first binding domain binds to a human CD3ε polypeptide or a cynomolgus monkey (cyno) CD3ε polypeptide.
4. The bispecific antibody of claim 1 , wherein the bispecific antibody comprises a substitution mutation in the Fc region that reduces effector function.
5. The bispecific antibody of claim 1 , wherein the bispecific antibody comprises one or more substitution mutations substitution mutation is at amino acid residue N297, L234, L235, and/or D265 (EU numbering).
6. The bispecific antibody of claim 5 , wherein the one or more substitution mutations comprises N297G, N297A, L234A, L235A, and/or D265A.
7. The bispecific antibody of claim 4 , wherein the substitution mutation is an aglycosylation site mutation.
8. The bispecific antibody of claim 7 , wherein the aglycosylation site mutation is at amino acid residue N297 (EU numbering).
9. The bispecific antibody of claim 8 , wherein the aglycosylation site mutation is N297G or N297A.
10. The bispecific antibody of claim 1 , wherein the bispecific antibody comprises a first CH1 (CH1 1 ) domain, a first CH2 (CH2 1 ) domain, a first CH3 (CH3 1 ) domain, a second CH1 (CH1 2 ) domain, a second CH2 (CH2 2 ) domain, and a second CH3 (CH3 2 ) domain, wherein
the CH3 1 domain is paired with the CH3 2 domain, wherein the CH3 1 and CH3 2 domains each comprise a knob or hole, and wherein the knob or hole in the CH3 1 domain is positionable in the hole or knob, respectively, in the CH3 2 domain.
11. The bispecific antibody of claim 1 , wherein the bispecific antibody is monoclonal, humanized, or chimeric.
12. The bispecific antibody of claim 1 , wherein the bispecific antibody is an antibody fragment that binds HER2 and CD3.
13. The bispecific antibody of claim 1 , wherein the bispecific antibody is a full-length antibody.
14. A composition comprising the bispecific antibody of claim 1 .
15. A kit comprising:
(a) the composition of claim 14 ; and
(b) a package insert comprising instructions for administering the composition to a subject to treat or delay progression of a cell proliferative disorder.
16. A bispecific antibody that binds to HER2 and CD3, wherein the bispecific antibody comprises an anti-HER2 arm comprising a first binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO: 270 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO: 271, and an anti-CD3 arm comprising a second binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO: 184 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO: 185.
17. A bispecific antibody that binds to HER2 and CD3, wherein the bispecific antibody comprises an anti-HER2 arm comprising a first binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO: 270 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO: 271, and an anti-CD3 arm comprising a second binding domain comprising (a) a VH domain comprising the amino acid sequence of SEQ ID NO: 184 and (b) a VL domain comprising the amino acid sequence of SEQ ID NO: 185, wherein the bispecific antibody comprises the substitution mutation N297G; and wherein:
(a) the anti-HER2 arm further comprises a CH1 1 domain, a CH2 1 domain, a CH3 1 domain;
(b) the anti-CD3 arm further comprises a CH1 2 domain, a CH2 2 domain, and a CH3 2 domain, wherein the CH3 1 domain is paired with the CH3 2 domain; and
(c) the CH3 1 domain comprises a knob, the CH3 2 domain comprises a hole, and the knob is positionable in the hole.
18. A kit comprising:
(a) the composition of claim 14 ; and
(b) a package insert comprising instructions for administering the composition to a subject to treat or delay progression of a HER2-positive cancer.