Devices, systems and methods for inhibiting invasion and metastases of cancer
The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.
1. A method comprising:
1) providing
a) a microfluidic device comprising:
a body having a first channel and a first chamber, an at least partially porous membrane positioned at an interface region between the first channel and the first chamber, the membrane comprising a top surface and a bottom surface, said top surface facing the first chamber;
living epithelial cells disposed within the first chamber as a layer of cells; and
living tumor cells in contact with said living epithelial cells, said tumor cells originating from a biopsy, and disposed within at least one of the first chamber or the first channel; and
2) testing said epithelial cell layer for invasion by said tumor cells.
2. The method of claim 1 , wherein the first chamber comprises a second channel.
3. The method of claim 1 , wherein the first chamber comprises an open region.
4. The method of claim 1 , wherein said tumor cells comprise melanoma cells.
5. The method of claim 1 , wherein the method further comprises endothelial cells disposed within the first channel.
6. A method comprising:
1) providing
a) a microfluidic device comprising a body having a first chamber, an at least partially porous membrane comprising a top surface and a bottom surface, said top surface facing the first chamber;
b) living epithelial cells disposed within the first chamber as a layer of cells; and
c) living tumor cells in contact with said living epithelial cells, and disposed within said first chamber; and
2) testing said epithelial cell layer for invasion by said tumor cells.
7. The method of claim 6 , wherein said tumor cells comprise melanoma cells.