SYSTEMS AND METHODS FOR ELECTROCHEMICAL CREATININE ASSAYS
A system for the electrochemical detection of creatinine levels includes a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine.
1 . A system for the electrochemical detection of creatinine levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area; and
a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine.
2 . The system of claim 1 , wherein the reagent coating includes a surfactant, a binder, stabilizers, a buffer, sarcosine dehydrogenase, and potassium ferricyanide.
3 . The system of claim 1 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.
4 . The system of claim 3 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
5 . The system of claim 4 , wherein the reagent coating includes a surfactant and a buffer.
6 . The system of claim 5 , wherein the reagent buffer includes a binder and a stabilizer.
7 . The system of claim 1 , wherein the reagent coating includes creatinine deiminase and a mediator.
8 . The system of claim 7 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
9 . The system of claim 1 , wherein the reagent coating includes sarcosine oxidase and a mediator.
10 . The system of claim 9 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
11 . A system for the electrochemical detection of creatinine levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area;
a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine; and
an analyzer for receiving the test strip and including instructions stored on a non-transitory medium for applying a current to the test strip and responsively determining an amount of creatinine.
12 . The system of claim 11 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.
13 . The system of claim 12 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
14 . The system of claim 13 , wherein the reagent coating includes a surfactant and a buffer.
15 . The system of claim 14 , wherein the reagent buffer includes a binder and a stabilizer.
16 . The system of claim 11 , wherein the reagent coating includes creatinine deiminase and a mediator.
17 . The system of claim 16 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
18 . A system for the electrochemical detection of creatinine levels, the system comprising:
a test strip including an electrode and a counter electrode, the electrode and counter electrode located proximate to a sample reception area;
a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine; and
an analyzer for receiving the test strip and including instructions stored on a non-transitory medium for determining a voltage of the test strip and responsively determining an amount of creatinine.
19 . The system of claim 18 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.
20 . The system of claim 19 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
21 . The system of claim 18 , wherein the reagent coating includes creatinine deiminase and a mediator.
22 . The system of claim 21 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
23 . A method of detecting creatinine, the method comprising:
providing an electrochemical test strip;
placing the electrochemical test strip in an analyzer;
placing a blood sample on the electrochemical test strip;
measuring a current provided through the blood sample and the electrochemical test strip; and
calculating a level of creatinine with the analyzer based on the current.
24 . The method of claim 18 , wherein the test strip includes an electrode and a counter electrode, the electrode and counter electrode located in a sample reception area; and a coating on one of the electrode and counter electrode, the coating including a reagent coating for creatinine.
25 . The method of claim 24 , wherein the reagent coating includes sarcosine dehydrogenase and a mediator.
26 . The method of claim 25 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.
27 . The method of claim 24 , wherein the reagent coating includes creatinine deiminase and a mediator.
28 . The method of claim 27 , wherein the mediator is selected from the list consisting of methylene blue, meldora blue, phenazine methosulfate, 2,6-Diclorophenol indophenol, nile blue, and potassium ferricyanide.