Insecticidal cry toxins
Insecticidal toxins derived from Bacillus thuringiensis , polynucleotides encoding such toxins, use of such toxins to control Coleopteran plant pests, and transgenic plants that produce, and are protected, by these toxins are described.
1. A nucleic acid construct comprising, a nucleic acid sequence encoding an insecticidal IRDIG protein comprising a sequence having at least 99% sequence identity with any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122, and a heterologous regulatory element.
2. A nucleic acid construct comprising a nucleic acid sequence of claim 1 encoding an insecticidal IRDIG protein comprising any one of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 71, 73, 75, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, and 122.
3. A plant or plant part comprising the construct of claim 1 .
4. The plant part of claim 3 wherein the plant part is a seed.
5. A plant or plant part comprising the construct of claim 2 .
6. The plant part of claim 5 wherein the plant part is a seed.
7. A plant or plant part comprising an insecticidal IRDIG protein encoded by the construct of claim 1 .
8. The plant part of claim 7 wherein the plant part is a seed.
9. A plant or plant part comprising an insecticidal IRDIG protein encoded by the construct of claim 2 .
10. The plant or plant part of claim 7 , wherein the insecticidal IRDIG protein has insecticidal activity against an insect selected from the group consisting of the order Coleoptera and Western Corn Rootworm.
11. The seed of claim 8 wherein the insecticidal IRDIG protein has insecticidal activity against an Coleoptera or Western Corn Rootworm insects.
12. A method for producing a coleopteran-tolerant plant comprising breeding a non-transgenic plant with a transgenic plant comprising the construct of claim 1 , stably incorporated into the genome of the plant, and selecting one or more progeny plants by analyzing for at least a portion of the construct in the one or more selected progeny plants.
13. A method for producing a coleopteran-tolerant plant comprising breeding a non-transgenic plant with a transgenic plant comprising the construct of claim 2 , stably incorporated into the genome of the plant, and selecting one or more progeny plants by analyzing for at least a portion of the construct in the selected one or more progeny plants.