METHOD FOR DETECTING SOLUBLE OLIGOMERIC AMYLOID BETA
A method for detecting soluble oligomeric amyloid β in a subject using, e.g., PET is provided.
1 . A kit for detecting soluble oligomeric amyloid β comprising
(a) an antibody or fragment thereof that selectively binds soluble oligomeric amyloid β, and either
(b) a labeling moiety selected from the group of:
(i) a chelating agent, and
(ii) a fluorescent label; or
(c) a labeled secondary antibody.
2 . The kit of claim 1 , further comprising a radioisotope.
3 . The kit of claim 1 , further comprising a standard curve.
4 . A method for quantitatively detecting soluble oligomeric amyloid β in a biological sample comprising
(a) contacting a biological sample from a subject comprising soluble oligomeric amyloid β with an antibody or fragment thereof exhibiting selective cross-reactivity with soluble oligomeric amyloid β over monomer and fibrillar amyloid; and
(b) quantitatively measuring binding of the antibody or fragment thereof to the soluble oligomeric amyloid β to obtain a concentration of soluble oligomeric amyloid β in the biological sample thereby quantitatively detecting soluble oligomeric amyloid β in the biological sample.
5 . The method of claim 4 , further comprising (c) correlating the concentration of soluble oligomeric amyloid β with the subject's cognitive function.
6 . The method of claim 4 , wherein the antibody is a monoclonal antibody or fragment thereof.
7 . The method of claim 4 , wherein the antibody is a humanized monoclonal antibody or fragment thereof.