Plant promoter and 3′UTR for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G144030 promoter. Some embodiments relate to a Zea mays GRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G144030 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a promoter operably linked to a heterologous polynucleotide sequence of:
a) a polylinker sequence;
b) a non-GRMZM2G144030 gene; or
c) a combination of a) and b), wherein said promoter comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1.
2. The nucleic acid vector of claim 1 , wherein said promoter is 2,082 bp in length.
3. The nucleic acid vector of claim 1 , wherein said promoter consists of a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1.
4. The nucleic acid vector of any one of claims 1 - 3 , further comprising a sequence encoding a selectable marker.
5. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a transgene.
6. The nucleic acid vector of claim 5 , wherein the transgene encodes a selectable marker or a gene product conferring insecticidal resistance, herbicide tolerance, nitrogen use efficiency, small RNA expression, site specific nuclease, water use efficiency, nutritional quality or expression of a DNA binding protein.
7. The nucleic acid vector of any of claim 1 - 3 , or 5 , further comprising a 3′ untranslated polynucleotide sequence that has at least 90% sequence identity with SEQ ID NO:5, wherein the 3′ untranslated sequence is operably linked to said polylinker or said non-GRMZM2G144030 gene.
8. The nucleic acid vector of any of claim 1 - 3 , or 5 , further comprising a 5′ untranslated polynucleotide sequence, wherein the 5′ untranslated sequence is operably linked to said polylinker or said non-GRMZM2G144030 gene.
9. The nucleic acid vector of any of claim 1 - 3 , or 5 , further comprising an intron sequence.
10. The nucleic acid vector of claim 1 , wherein said promoter drives transgene expression in leaf, root, stem, cob, kernel, pollen and silk tissues.
11. A non- Zea mays c.v. B73 plant comprising a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1 operably linked to a transgene, wherein the polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1 drives expression of the transgene.
12. The plant of claim 11 , wherein said plant is selected from the group consisting of wheat, rice, sorghum, oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.
13. The plant of claim 11 , wherein said plant is maize.
14. The plant of any one of claims 11 - 13 , wherein the polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1 operably linked to the transgene is inserted into the genome of said plant.
15. The plant of claim 11 , further comprising a 3′ untranslated sequence comprising SEQ ID NO:5, wherein the 3′ untranslated sequence is operably linked to said transgene.
16. The plant of claim 11 , wherein said promoter drives transgene expression root, stem, cob, kernel and silk tissues.
17. The plant of claim 11 , wherein said promoter is 2,082 bp in length.
18. A method for producing a transgenic plant cell, the method comprising the steps of:
a) transforming a plant cell with a gene expression cassette comprising a Zea mays GRMZM2G144030 promoter, wherein said Zea mays GRMZM2G144030 promoter comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1, operably linked to at least one polynucleotide sequence of interest;
b) isolating the transformed plant cell comprising the gene expression cassette; and,
c) producing a transgenic plant cell comprising the Zea mays GRMZM2G144030 promoter operably linked to at least one polynucleotide sequence of interest.
19. A method for expressing a polynucleotide sequence of interest in a plant cell, the method comprising introducing into the plant cell a polynucleotide sequence of interest operably linked to a Zea mays GRMZM2G144030 promoter, wherein said Zea mays GRMZM2G144030 promoter comprises a polynucleotide sequence that has at least 97% sequence identity with SEQ ID NO:1.