Plant promoter and 3'UTR for transgene expression
This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.
1. A nucleic acid vector comprising a promoter operably linked to a heterologous polynucleotide sequence of:
a) a polylinker sequence;
b) a non-GRMZM2G047720 gene; or
c) a combination of a) and b), wherein said promoter consists of the polynucleotide sequence of SEQ ID NO:1, wherein said promoter drives leaf, stem, cob, kernel, pollen and silk tissue preferred expression.
2. The nucleic acid vector of claim 1 , wherein said promoter is 2,065 bp in length.
3. The nucleic acid vector of any one of claims 1 - 2 , further comprising a sequence encoding a selectable marker.
4. The nucleic acid vector of claim 1 , wherein said promoter is operably linked to a transgene.
5. The nucleic acid vector of claim 4 , wherein the transgene encodes a selectable marker or a gene product conferring insecticidal resistance, herbicide tolerance, nitrogen use efficiency, small RNA expression, site specific nuclease, water use efficiency, nutritional quality or DNA binding proteins.
6. The nucleic acid vector of any of claim 1 - 2 , or 4 , further comprising a 3′ untranslated polynucleotide sequence, wherein the 3′ untranslated sequence is operably linked to said polylinker or said transgene.
7. The nucleic acid vector of any of claim 1 - 2 , or 4 , further comprising a 5′ untranslated polynucleotide sequence, wherein the 5′ untranslated sequence is operably linked to said polylinker or said transgene.
8. The nucleic acid vector of any of claim 1 - 2 , or 4 , further comprising an intron sequence.
9. A non- Zea mays c.v. B73 plant comprising a promoter, wherein said promoter consists of the polynucleotide sequence of SEQ ID NO:1 operably linked to a transgene, wherein said promoter drives leaf, stem, cob, kernel, pollen and silk tissue preferred expression.
10. The plant of claim 9 , wherein said plant is selected from the group consisting of wheat, rice, sorghum, oats, rye, bananas, sugar cane, soybean, cotton, Arabidopsis , tobacco, sunflower, and canola.
11. The plant of claim 9 , wherein said plant is maize.
12. The plant of claim 9 , further comprising a 3′ untranslated sequence comprising SEQ ID NO:5, wherein the 3′ untranslated sequence is operably linked to said transgene.
13. The plant of claim 9 , wherein said promoter is 2,065 bp in length.
14. A method for producing a transgenic plant cell, the method comprising the steps of:
a) transforming a plant cell with a gene expression cassette comprising a Zea mays GRMZM2G047720 promoter, wherein said promoter consists of the polynucleotide sequence SEQ ID NO:1, operably linked to at least one polynucleotide sequence of interest;
b) isolating the transformed plant cell comprising the gene expression cassette; and,
c) producing a transgenic plant cell comprising the Zea mays GRMZM2G047720 promoter operably linked to at least one polynucleotide sequence of interest, wherein said promoter drives leaf, stem, cob, kernel, pollen and silk tissue preferred expression.
15. A method for expressing a polynucleotide sequence of interest in cob and pollen tissue of a plant cell, the method comprising introducing into the plant cell a polynucleotide sequence of interest operably linked to a Zea mays GRMZM2G047720 promoter, wherein said promoter comprises the polynucleotide sequence of SEQ ID NO:1.