SOLUTIONS FOR SAMPLE PROCESSING
This disclosure is directed to solutions and methods of using the solutions. A first solution includes a polymer, a polyol, and a buffer. A second solution includes a polymer, a polyol, and an alcohol.
1 . A solution comprising:
a polyol having a concentration of 5-30% by mass;
polyvinyl alcohol (“PVA”) having a concentration of 5-20% by mass; and
a buffer.
2 . The solution of claim 1 , wherein the polyol is glycerol, propylene glycol, or pentaerythritol.
3 . The solution of claim 2 , wherein the buffer is tris-EDTA buffer (“TE buffer”) or a phosphate buffer.
4 . The solution of claim 1 , wherein the buffer is TE buffer or a phosphate buffer.
5 . The solution of claim 1 , wherein the polyol is glycerol and has a concentration of 10% by mass, wherein the PVA has a concentration of 10% by mass, wherein the buffer is TE buffer and the tris has a concentration of 20 mM and the EDTA has a concentration of 2 mM.
6 . A system comprising:
a solution comprising
a polyol having a concentration of 5-30% by mass,
polyvinyl alcohol (“PVA”) having a concentration of 5-20% by mass, and a buffer; and
a set of stains comprising
at least one quantum dot, and
at least one fluorophore.
7 . The system of claim 6 , wherein the polyol is glycerol, propylene glycol, or pentaerythritol.
8 . The system of claim 7 , wherein the buffer is tris-EDTA buffer (“TE buffer”) or a phosphate buffer.
9 . The system of claim 6 , wherein the buffer is TE buffer or a phosphate buffer.
10 . The system of claim 6 , wherein the polyol is glycerol and has a concentration of 10% by mass, wherein the PVA has a concentration of 10% by mass, wherein the buffer is TE buffer and the tris has a concentration of 20 mM and the EDTA has a concentration of 2 mM.
11 . A method comprising:
providing a sample comprising a target material;
labeling the target material with a set of stains comprising
at least one quantum dot, and
at least one fluorophore; and
covering the sample with a solution comprising
a polyol having a concentration of 5-30% by mass,
polyvinyl alcohol (“PVA”) having a concentration of 5-20% by mass, and a buffer,
wherein the covering step occurs after the labeling step.
12 . The method of claim 11 , further comprising the step of:
imaging the sample,
wherein the imaging step occurs after the covering step.
13 . The method of claim 12 , wherein the set of stains retains at least 75% emission after 24 hours of contact with the solution, and wherein 100% emission is the emission of the set of stains when the sample is imaged without the solution.
14 . The method of claim 13 , wherein the polyol is glycerol, propylene glycol, or pentaerythritol.
15 . The method of claim 14 , wherein the buffer is tris-EDTA buffer (“TE buffer”) or a phosphate buffer.
16 . The method of claim 12 , wherein the set of stains retains at least 50% emission after 72 hours of contact with the solution, and wherein 100% emission is the emission of the set of stains when the sample is imaged without the solution.
17 . The method of claim 16 , wherein the polyol is glycerol, propylene glycol, or pentaerythritol.
18 . The method of claim 17 , wherein the buffer is tris-EDTA buffer (“TE buffer”) or a phosphate buffer.
19 . The method of claim 11 , wherein the buffer is TE buffer or a phosphate buffer.
20 . The method of claim 11 , wherein the polyol is glycerol and has a concentration of 10% by mass, wherein the PVA has a concentration of 10% by mass, wherein the buffer is TE buffer and the tris has a concentration of 20 mM and the EDTA has a concentration of 2 mM.