IP Library › Patent Application 15493806
Patent Application
App. No. 15/493,806

METHOD FOR CONTROLLING STREPTOCOCCUS PNEUMONIAE POLYSACCHARIDE MOLECULAR WEIGHT USING CARBON DIOXIDE

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Patent No.
US None
App. No.
15/493,806
Abstract

The present invention provides improved methods for producing a solution containing high molecular weight isolated Streptococcus pneumoniae capsular polysaccharides having phosphodiester linkages between saccharide repeat units. In certain methods, CO 2 is supplied to a fermentation culture of Streptococcus pneumoniae bacterial cells that produce capsular polysaccharide serotypes containing phosphodiester linkages between saccharide repeat units. Exemplary Streptococcus pneumoniae serotypes containing a phosphodiester linkage between saccharide repeat units include serotypes 6A, 6B, 19A, and 19F. Supplying CO 2 to the fermentation culture includes adding bicarbonate ions to the fermentation culture, adding carbonate ions to the fermentation culture, adding mixtures of bicarbonate and carbonate ions to the fermentation culture, and overlaying the fermentation culture with CO 2 .

Claims (5)

1 . A solution containing high molecular weight isolated Streptococcus pneumoniae capsular polysaccharides, wherein said Streptococcus pneumoniae capsular polysaccharides are serotype 6A, and, wherein the molecular weight of said isolated Streptococcus pneumoniae capsular polysaccharide is at least 700 kDa.

2 . The solution according to claim 1 wherein the molecular weight of said isolated Streptococcus pneumoniae capsular polysaccharide is at least 900 kDa.

3 . The solution according to claim 2 wherein the molecular weight of said isolated Streptococcus pneumoniae capsular polysaccharide is at least 1000 kDa.

4 . A solution containing high molecular weight isolated Streptococcus pneumoniae capsular polysaccharides, wherein said Streptococcus pneumoniae capsular polysaccharides are serotype 19A, and, wherein the molecular weight of said isolated Streptococcus pneumoniae capsular polysaccharide is at least 480 kDa.

5 . The solution according to claim 4 wherein the molecular weight of said isolated Streptococcus pneumoniae capsular polysaccharide is at least 700 kDa.