IP Library › Patent Application 15502129
Patent Application
App. No. 15/502,129

PLANT REGULATORY ELEMENTS AND METHODS OF USE THEREOF

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Quick Facts
Patent No.
US None
App. No.
15/502,129
Abstract

The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for regulatory elements from Lamium Leaf Distortion Associated Virus. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory element sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the regulatory elements of the present disclosure.

Claims (84)

1 . A recombinant polynucleotide selected from:

(a) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 1 or,

(b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 1,

(c) the polynucleotide of SEQ ID NO: 1, and

(c) a fragment of SEQ ID NO: 1.

2 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide has at least 90 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 1.

3 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide has at least 95 percent sequence identity to a nucleic acid sequence of SEQ ID NO: 1.

4 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide is selected from:

(a) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 2 or,

(b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 2,

(c) the polynucleotide of SEQ ID NO: 2, and

(c) a fragment of SEQ ID NO: 2.

5 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide is selected from:

(a) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 3 or,

(b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 3,

(c) the polynucleotide of SEQ ID NO: 3, and

(c) a fragment of SEQ ID NO: 3.

6 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide is selected from:

(a) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 4 or,

(b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 4,

(c) the polynucleotide of SEQ ID NO: 4, and

(c) a fragment of SEQ ID NO: 4.

7 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide is selected from:

(a) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 5 or,

(b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 5,

(c) the polynucleotide of SEQ ID NO: 5, and

(c) a fragment of SEQ ID NO: 5.

8 . The recombinant polynucleotide of claim 1 , wherein the polynucleotide is selected from:

(a) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 6 or,

(b) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 6,

(c) the polynucleotide of SEQ ID NO: 6, and

(c) a fragment of SEQ ID NO: 6.

9 . A DNA construct; comprising

(a) a regulatory element polynucleotide selected from the group consisting of:

(i) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 1,

(ii) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 1

(iii) the polynucleotide of SEQ ID NO: 1, and

(iv) a fragment of SEQ ID NO: 1; and

(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide.

10 . The DNA construct of claim 9 , wherein the regulatory element polynucleotide has at least 90 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 1.

11 . The DNA construct of claim 9 , wherein the regulatory element polynucleotide has at least 95 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 1.

12 . The DNA construct of claim 9 ; comprising

(a) a regulatory element polynucleotide selected from the group consisting of:

(i) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 2,

(ii) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 2

(iii) the polynucleotide of SEQ ID NO: 2, and

(iv) a fragment of SEQ ID NO: 2; and

(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide.

13 . The DNA construct of claim 9 ; comprising

(a) a regulatory element polynucleotide selected from the group consisting of:

(i) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 3,

(ii) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 3

(iii) the polynucleotide of SEQ ID NO: 3, and

(iv) a fragment of SEQ ID NO: 3; and

(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide.

14 . The DNA construct of claim 9 ; comprising

(a) a regulatory element polynucleotide selected from the group consisting of:

(i) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 4,

(ii) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 4

(iii) the polynucleotide of SEQ ID NO: 4, and

(iv) a fragment of SEQ ID NO: 4; and

(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide.

15 . The DNA construct of claim 9 ; comprising

(a) a regulatory element polynucleotide selected from the group consisting of:

(i) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 5,

(ii) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 5

(iii) the polynucleotide of SEQ ID NO: 5, and

(iv) a fragment of SEQ ID NO: 5; and

(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide.

16 . The DNA construct of claim 9 ; comprising

(a) a regulatory element polynucleotide selected from the group consisting of:

(i) a polynucleotide having at least 85 percent sequence identity to the nucleic acid sequence of SEQ ID NO: 6,

(ii) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 6

(iii) the polynucleotide of SEQ ID NO: 6, and

(iv) a fragment of SEQ ID NO: 6; and

(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element polynucleotide.

17 . The DNA construct of claim 9 , wherein the heterologous transcribable polynucleotide molecule is a gene of agronomic interest.

18 . The DNA construct of claim 17 , wherein the heterologous transcribable polynucleotide molecule is a gene capable of providing herbicide resistance in plants.

19 . The DNA construct of claim 17 , wherein the heterologous transcribable polynucleotide molecule is a gene capable of providing plant pest control in plants.

20 . A heterologous cell stably transformed with the nucleic acid molecule of claim 1 .

21 . A transgenic plant or plant cell stably transformed with the DNA construct of claim 9 .

22 . The transgenic plant or plant cell of claim 21 , wherein the transgenic plant is a dicotyledon plant cell.

23 . The transgenic plant or plant cell of claim 21 , wherein the transgenic plant is a monocotyledon plant cell.

24 . A seed of the transgenic plant of claim 21 , wherein the seed comprises the DNA construct.