Method and kit for detecting bacterial infection
The invention relates to a novel antibody which binds to C-SLPI selected from the group consisting of SEQ ID NOs: 3 and 4.
1. An immunogen comprising a polypeptide hapten of 5-12 amino acids in length comprising the structure Gln-Cys-Leu-Arg (SEQ ID NO: 10), wherein the N-terminus region of the polypeptide hapten comprises SEQ ID NO: 10; and wherein the polypeptide hapten is linked to an immunogenicity conferring carrier molecule by the C-terminal amino acid of the polypeptide hapten.
2. An antibody, or antigen binding fragment thereof, that is capable of specifically binding, to an epitope of C-terminal peptide fragment of Secretory leukocyte protease inhibitor (C-SLPI), the peptide fragment which is 10, 11 or 12 amino acids in length and comprises SEQ ID NO: 6 wherein the N-terminus region of the polypeptide fragment comprises SEQ ID NO: 6 and wherein the cross-reactivity of the antibody with full-length SLPI is <0.05%.
3. A method of determining an individual's C-SLPI level by:
(a) contacting an antibody specific for C-terminal fragment of Secretory leukocyte protease inhibitor (C-SLPI) with a sample from the individual; and
(b) detecting the amount of C-terminal fragment of Secretory leukocyte protease inhibitor (C-SLPI) in the sample;
wherein the cross-reactivity of the antibody with full-length Secretory leukocyte protease inhibitor (SLPI) is <0.05%.