Method and apparatus for rapid sequential flow injection
View Patent ↗A fluid delivery system and process for fluid delivery is provided that is useful in in flow-injection based sensing measurements, wherein samples from a sample rack are introduced into a flow cell where sensing measurements are taken. The system and process utilize at least two holding lines where each holding line can retain one of the samples prior to injection of the thus retained sample into a flow cell. The introduction of the samples from the sample racks to the holding lines is alternated and the injection of the samples from the holding lines to the flow cell are alternated so that one holding line is loaded with one of the samples simultaneously with another sample being injected from the another holding line to the flow cell.
1. A fluid delivery system for an apparatus for making flow-injection based sensing measurements comprising:
a flow cell from which sensing measurements can be taken;
a first holding line;
a second holding line;
a probe through which a plurality of samples are introduced into the fluid delivery system;
a first multi-port valve having a first position and a second position, wherein the first multi-port valve is in fluid flow communication with the probe, the first holding line, and the second holding line; and
a second multi-port valve having a first position and a second position, wherein the second multi-port valve is in fluid flow communication with the first multi-port valve and the flow cell; and
wherein, when the first multi-port valve is in the first position and the second multi-port valve is in the second position, one of the samples can be introduced from the first holding line to the flow cell simultaneously with another of the samples being introduced from the probe to the second holding line; and, when the first multi-port valve is in the second position and the second multi-port valve is in the second position, one of the samples can be introduced from the second holding line to the flow cell simultaneously with another of the samples being introduced from the probe to the first holding line.
2. The fluid delivery system of claim 1 , further comprising:
a first pump in fluid flow communication to a buffer storage and the first holding line; and
a second pump in fluid flow communication to the buffer storage and the second holding line.
3. The fluid delivery system of claim 1 , further comprising:
a buffer storage;
a first buffer line in fluid flow communication with the buffer storage and the second multi-port valve such that the second valve can receive buffer solution from the first buffer line and, when the second valve is in the first position, the second valve can introduce the buffer solution to the flow cell and, when the second valve is in the second position, the second valve can introduce buffer solution to the probe; and
a second buffer line in fluid flow communication with the buffer storage and the second multi-port valve that the second valve can receive buffer solution from the second buffer line and, when the second valve is in the first position, the second valve can introduce the buffer solution to the flow cell and, when the second valve is in the second position, the second valve can introduce buffer solution to the probe.
4. The fluid delivery system of claim 3 , further comprising:
a first pump in fluid flow communication to the buffer storage, the first holding line and the first buffer line; and
a second pump in fluid flow communication to the buffer storage, the second holding line and the second buffer line.
5. The fluid delivery system of claim 4 , wherein:
when the first multi-port valve is in the first position and the second multi-port valve is in the first position, the first pump can provide buffer fluid from the buffer storage to the probe through first holding line, the first multi-port valve and the second multi-port valve; and
when the first multi-port valve is in the second position and the second multi-port valve is in the first position, the second pump can provide buffer fluid from the buffer storage to the probe through the second holding line, the first multi-port valve and the second multi-port valve.
6. The fluid delivery system of claim 5 , further comprising:
a third buffer line in fluid flow communication with the first pump and the first multi-port valve such that, when the first multi-port valve is in the first position, the first pump can introduce buffer solution from the buffer storage to the probe through the first multi-port valve; and
a fourth buffer line in fluid flow communication with the second pump and the first multi-port valve such that, when the first multi-port valve is in the second position, the second pump can introduce buffer solution from the buffer storage to the probe through the first multi-port valve.
7. The process of claim 6 , further comprising a dispersion loop situated between the first multiport valve and the second multiport valve, wherein the samples pass through the dispersion loop and the dispersion loop forms a gradient in concentration of the sample prior to injection of the sample to the flow cell.