Method for the production of yeast
View Patent ↗The invention relates to the fields of industrial microbiology and alcohol production including production of yeast products with features suitable for transport, storage, and utilization in fermentation.
1. A method of producing a yeast product comprising:
a) culturing a population of yeast in the presence of a carbon substrate, nitrogen substrate, and ethanol whereby the feed rate of carbon substrate and ethanol is ramped such that concentration of carbon substrate and ethanol maintains the growth rate of the population at less than μ crit and whereby the population reaches a cell density;
b) ceasing the ramping of the feed rate whereby the concentration of carbon substrate, nitrogen substrate, and ethanol decreases over a period of time; and
c) subjecting the population of yeast to a feed rate which is ramped down to less than half of the maximum feed rate of (a).
2. The method of claim 1 , wherein the carbon substrate is glucose, acetate, or a mixture thereof.
3. The method of claim 1 , wherein the carbon substrate is a dual carbon source.
4. The method of claim 3 , wherein the dual carbon source is glucose and acetate.
5. The method of claim 1 , wherein the yeast is a butanologen.
6. The method of claim 1 further comprising concentrating the population of yeast to at least about 16% (w/v).
7. The method of claim 1 further comprising concentrating the population of yeast to at least about 30% (w/v).
8. The method of claim 6 , wherein the concentrating is carried out in a centrifuge.
9. The method of claim 6 , wherein (b) and (c) increase the trehalose concentration of the population of yeast by at least 100%.
10. The method of claim 6 , wherein (b) and (c) increase the trehalose concentration of the population of yeast by at least 500%.
11. The method of claim 6 , wherein the trehalose content of the population of yeast is at least about 5%.
12. The method of claim 6 , wherein the population of yeast is further concentrated to at least about 90% (w/v).
13. The method of claim 12 , wherein the population of yeast is further concentrated to at least about 90% (w/v) by drying.
14. The method of claim 7 , wherein the concentrating is carried out in a centrifuge.
15. The method of claim 7 , wherein (b) and (c) increase the trehalose concentration of the population of yeast by at least 100% or by at least 500%.
16. The method of claim 7 , wherein the population of yeast is further concentrated to at least about 90% (w/v).