IP Library › Granted Patent US 10,421,791
Granted Patent B2
US 10,421,791 · App. 15/506,320 · Granted Sep 24, 2019

Modified Vip3 polypeptides

Inventor: Joseph M. Watts (Research Triangle Park, NC)
Assignee: Syngenta Participations AG
C07K14/32A01N37/46A01N63/02C07K14/195C07K14/325C12N15/8279C12N15/8286C07K2319/20C07K2319/55Y02A40/162
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Quick Facts
Patent No.
US 10,421,791
App. No.
15/506,320
Granted
Sep 24, 2019
Kind
B2
Abstract

The present invention is directed to vegetative insecticidal proteins (Vips) modified to comprise heterologous carbohydrate binding modules and methods of use thereof.

Claims (9)

1. A modified Vip3 polypeptide comprising a heterologous carbohydrate binding module (CBM), wherein the CBM is from a bacterial β-1,4-mannanase, wherein the heterologous CBM is substituted for all or a portion of Domain III of a Vip3 polypeptide, and wherein the modified Vip3 polypeptide is pesticidal against an insect.

2. The modified Vip3 polypeptide of claim 1 , wherein the Vip3 polypeptide comprises all or a portion of SEQ ID NO:1.

3. The modified Vip3 polypeptide of claim 1 , wherein all or a portion of Domain III comprises amino acids 542 to 667 of SEQ ID NO:1.

4. The modified Vip3 polypeptide of claim 1 , wherein the heterologous CBM comprises a metal binding site.

5. The modified Vip3 polypeptide of claim 1 , wherein the modified Vip3 polypeptide is pesticidal against a lepidopteran insect.

6. The modified Vip3 polypeptide of claim 1 , wherein the modified Vip3 polypeptide is pesticidal against at least one of Ostrinia nubilalis (European corn borer), Plutella xylostella (diamondback moth), Spodoptera frugiperda (fall armyworm), Agrotis ipsilon (black cutworm), Agrotis orthogonia (pale western cutworm), Striacosta albicosta (western bean cutworm), Helicoverpa zea (corn earworm), Heliothis virescens (tobacco budworm), Spodoptera exigua (beet armyworm), Helicoverpa punctigera (native budworm), Helicoverpa armigera (cotton bollworm), Manduca sexta (tobacco hornworm), Trichoplusia ni (cabbage looper), Pectinophora gossypiella (pink bollworm), Diatraea grandiosella (southwestern corn borer), Diatraea saccharalis (sugarcane borer), Elasmopalpus lignosellus (lesser cornstalk borer), Psuedoplusia includens (soybean looper), Anticarsia gemmatalis (velvetbean caterpillar), Plathypena scabra (green cloverworm), Homoeosoma electellum (sunflower head moth), and Cochylis hospes (banded sunflower moth), or any combination thereof.

7. A composition comprising the modified Vip3 polypeptide of claim 1 in an agriculturally acceptable carrier.

8. The modified Vip3 polypeptide of claim 1 , wherein the heterologous CBM is SEQ ID NO:24.

9. The modified Vip3 polypeptide of claim 1 , wherein the modified Vip3 polypeptide comprises SEQ ID NO:7.

Assignments (2)
MERGER AND CHANGE OF NAME Recorded Mar 14, 2025
From: SYNGENTA PARTICIPATIONS AG; SYNGENTA PARTICIPATIONS AG
To: SYNGENTA CROP PROTECTION AG
Reel/Frame 070517/0697 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 31, 2018
From: WATTS, JOSEPH M.
To: SYNGENTA PARTICIPATIONS AG
Reel/Frame 045945/0823 →
Continuity (2)
Provisional Application 62043922 · Aug 29, 2014
Related Publication 20170291925A1 · Oct 12, 2017
Cited By (1)
US 12,207,658