SYSTEM AND METHOD FOR INTRAOPERATIVE CELL STORAGE, PROCESSING, AND IMAGING

1 . A system for storing and processing one or more target cells comprising:

a. A collection container including a first chamber for receiving a tissue and a second chamber for processing the tissue into a plurality of cells, a media inlet for introducing a culture media to the collection container, a gas inlet for introducing gas from a gas controller to the collection container, an attached humidity and temperature controller for controlling the humidity and temperature of the collection container;

b. A controllable separator separating the first chamber from the second chamber;

c. A tissue inlet in the first chamber for receiving the tissue; and

d. A cell outlet in the second chamber connected to a fluidic device for separating one or more target cells from non-target cells.

2 . (canceled)

3 . The system as in claim 1 , wherein an internal surface of the collection container is coated with extracellular matrix.

4 . The system as in claim 1 , wherein the collection container includes a third chamber for receiving excess fluids and a controllable filter separating the second chamber from the third chamber.

5 . The system as in claim 1 , wherein the tissue inlet in the first chamber is connected to a tissue resector tool through a tissue collection tube.

6 . (canceled)

7 . The system as in claim 1 , further comprising one or more inlets in the second chamber for receiving a liquid saline solution and digestive enzymes.

8 . The system as in claim 1 , further comprising a movable means in the second chamber for dissociating the tissue.

9 . (canceled)

10 . (canceled)

11 . (canceled)

12 . (canceled)

13 . The system as in claim 1 , wherein a fluidic pump is connected to the fluidic device to propel the cells through the fluidic device.

14 . The system as in claim 1 , wherein the fluidic device comprises two or more channels for the cells.

15 . The system as in claim 1 , wherein the fluidic device comprises two or more channels for the cells, a temperature control plate, a single cell filter in at least one of the two or more channels, and one or more containers for receiving the target cells.

16 . The system as in claim 1 , wherein a laser is integrated in the fluidic device.

17 . The system as in claim 14 , wherein a laser is connected through a fiber bundle to at least one of the two or more channels of the fluidic device, the fiber bundle including excitation fibers to provide optical energy for cell movement between the two or more channels and to provide an optical spectra of the cells, and detection fibers to receive the emitted optical spectra.

18 . A system for storing and processing one or more target cells comprising:

a. A collection container including a first chamber for receiving a tissue, a second chamber for dissociating the tissue into a plurality of cells, a third chamber for receiving an excess fluid from the tissue, an inlet for introducing gas from a gas controller to the collection container, an attached humidity and temperature controller for controlling the humidity and temperature of the collection container;

b. A controllable separator to separate the first chamber from the second chamber;

c. A controllable filter to separate the second chamber from the third chamber;

d. A tissue inlet in the first chamber connected to a tissue resector tool through a collection tube;

e. A media inlet in the first chamber for receiving a culture medium;

f. One or more inlets in the second chamber for receiving a liquid saline solution and digestive enzymes;

g. A movable means in the second chamber for dissociating the tissue;

h. A cell outlet in the second chamber for the cells;

i. An excess fluid outlet in the third chamber connected to a container for storing the excess fluid;

j. A fluidic device connected to the cell outlet in the second chamber, the fluidic device comprising two or more channels for the cells, a temperature control plate, a fluidic pump to propel the cells through the channels, a single cell filter in at least one of the channels;

k. a laser connected through a fiber bundle to at least one of the channels of the fluidic device, the fiber bundle including excitation fibers to provide optical energy for cell movement between the channels and to provide an optical spectra of the cells, and detection fibers to receive the emitted optical spectra, and one or more containers for receiving the target cells; and

l. At least one control box connected to the gas controller, the humidity and temperature controller, the culture medium inlet, the one or more second chamber inlet, the cell outlet, the excess fluid outlet, the laser and the fluidic pump.

19 . A method for storing and processing one or more target cells comprising:

a. Receiving tissue into a collection chamber;

b. Maintaining the tissue in the collection chamber at a physiological temperature, humidity and atmosphere;

c. Passing the tissue from the collection chamber to a process chamber;

d. Dissociating the tissue into a plurality of single cells in the process chamber; and

e. Passing the single cells from the process chamber to a fluidic device for isolating one or more target cells.

20 . The method as in claim 19 , wherein the tissue is received intraoperatively from a tissue resector tool through a tissue collection tube.

21 . (canceled)

22 . The method as in claim 19 , wherein a culture media comprising one or more of: serum; epidermal growth factor; fibroblast growth factor 2; and extracellular matrix, is added to the tissue in the collection chamber.

23 . (canceled)

24 . (canceled)

25 . The method as in claim 19 , wherein digestive enzymes are added to the process chamber to dissociate the tissue into a plurality of cells.

26 . (canceled)

27 . The method as in claim 19 , wherein the tissue in the process chamber is mechanically stirred to dissociate the tissue into a plurality of single cells.

28 . (canceled)

29 . (canceled)

30 . (canceled)

31 . The method as in claim 19 , wherein the one or more target cells are isolated in the fluidic device by:

a. Directing optical waves at the single cells within the fluidic device to move the single cells into a channel within the fluidic device; and

b. Passing the single cells from the channel to a storage container.

32 . (canceled)

33 . (canceled)

34 . The method as in claim 31 , wherein the optical waves are provided by a laser connected to the fluidic device through a fiber bundle including excitation fibers for providing excitation optical waves and detection fibers for measuring emitted optical spectra from the single cells, and the measured spectra are used to identify the one or more target cells.

35 . The method as in claim 34 , wherein the one or more target cells are identified by comparing the spectra of the single cells to a database of spectra to determine whether the spectra of the single cells matches the spectra of the one or more target cells.

36 . (canceled)

37 . (canceled)