Centrifuge tube comprising a floating buoy, and methods for using the same
Multi-component separation devices configured to separate components of a liquid sample by centrifugation are provided. Aspects of the separation devices include a container having a distal end and a proximal end and a buoy configured to be displaced along a longitudinal axis within the container where the buoy includes one or more sealed chambers. Also provided are methods of using the subject devices to separate components of a multi-component liquid sample such as whole blood, bone marrow aspirate or stromal vascular fraction as well as systems suitable for practicing the subject methods.
1. A device for separating components of a multi-component liquid, the device comprising:
a container comprising a cavity, a distal end and a proximal end; and
a buoy having a distal end and a proximal end and is configured to be displaced along a longitudinal axis within the container, wherein the buoy comprises:
one or more chambers containing a vacuum or a fluid and sealed from fluidic communication with an outside environment of the buoy;
a concave first outer surface at the proximal end of the buoy, the first surface comprising a first orifice at the base of the concave outer surface, wherein the buoy does not comprise a valve at the first orifice;
a second outer surface at the distal end of the buoy comprising a second orifice at a position distal along the longitudinal axis of the buoy to the first orifice, the first orifice in fluid communication with the second orifice through a channel extending from the first orifice to the second orifice; and
a centrifuge activated ball and spring valve at the second orifice comprising an open position and a closed position;
wherein the valve is configured to be in the closed position to fluidically seal the second orifice without centrifugation, wherein the valve is configured to continuously be in the open position during centrifugation in response to a force of centrifugation, wherein the ball of the valve is configured to compress in response to the applied force of centrifugation in the open position.
2. The device according to claim 1 , wherein the container further comprises a cap positioned at the proximal end having one or more ports into the cavity of the container for passage of biological fluids.
3. The device according to claim 2 , further comprising a conduit that extends from the one or more ports to the buoy.
4. The device according to claim 3 , wherein the conduit further comprises a stream modulator that is configured to adjust the shape of fluid output from the conduit, the rate of fluid output from the conduit, or a combination thereof.
5. A device for separating components of a multi-component liquid, the device comprising:
a container comprising a cavity, a distal end and a proximal end; and
a buoy having a distal end and a proximal end and is configured to be displaced along a longitudinal axis within the container, wherein the buoy consists essentially of:
one or more chambers containing a vacuum or a fluid and sealed from fluidic communication with an outside environment of the buoy;
a concave first outer surface at the proximal end of the buoy, the first surface comprising a first orifice at the base of the concave outer surface, wherein the buoy does not comprise a valve at the first orifice;
a second outer surface at the distal end of the buoy comprising a second orifice at a position distal along the longitudinal axis of the buoy to the first orifice, the first orifice in fluid communication with the second orifice through a channel extending from the first orifice to the second orifice; and
a centrifuge activated valve at the second orifice comprising an open position and a closed position;
wherein the valve is configured to be in the closed position to fluidically seal the second orifice without centrifugation, wherein the valve is configured to continuously be in the open position during centrifugation in response to a force of centrifugation, wherein the ball of the valve is configured to compress the spring in response to an applied force of centrifugation in the open position.
6. The device of claim 1 , wherein the fluid is a gas or a liquid.