Tumor antigen peptide
The object is to provide a tumor antigen peptide that is specifically presented on a cancer and a cancer stem cell, and a pharmaceutical composition, etc. that is useful for the prevention and/or treatment of a cancer and contains the above peptide as an active ingredient. The above object has been accomplished by providing a BORIS-derived partial peptide belonging to isoform A or C or subfamily 5 or 6, a polynucleotide encoding the peptide, a pharmaceutical composition containing the above as an active ingredient, and an agent for the prevention and/or treatment of a cancer, the agent containing the above as an active ingredient and inducing CTLs.
1. A method for inducing a cytotoxic T cell (CTL) that specifically recognizes a cell expressing a BORIS gene belonging to isoform A or C or a BORIS gene belonging to subfamily 5 or 6, the method comprising contacting in vitro:
(a) a polypeptide comprising the amino acids of SEQ ID NO: 3, SEQ ID NO: 10, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67 or SEQ ID NO: 72, and having HLA-A11 antigen-binding capacity or HLA-A24 antigen-binding capacity, with a peripheral blood lymphocyte (PBL) expressing on the cell surface HLA-A11 antigen or HLA-A24 antigen,
(b) a polypeptide comprising the amino acids of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 10, SEQ ID NO: 47, or SEQ ID NO: 57, and having HLA-A2 antigen binding capacity, with a PBL expressing on the cell surface HLA-A2 antigen, or
(c) an expression vector encoding at least one of the polypeptides of (a) with the PBL expressing on the cell surface the HLA-A11 antigen or the HLA-A24 antigen or the polypeptide of (b) above with the PBL expressing on the cell surface the HLA-A2 antigen, wherein the sequence encoding the polypeptide of (a) or the polypeptide of (b) is operably linked to an expression control sequence:
wherein the polypeptide induces the CTL.
2. The method according to claim 1 , the polypeptide of (a) having the HLA-A11 antigen-binding capacity.
3. The method according to claim 1 , the polypeptide of (a) having the HLA-A24 antigen-binding capacity.
4. The method according to claim 1 , the step of contacting in vitro further comprising pulsing the PBL with the polypeptide.
5. The method according to claim 1 , the method further comprising intravenous, subcutaneous or intradermal administration of a pharmaceutical composition containing as an active ingredient the CTL to a patient having a cancer that is positive for the BORIS gene belonging to the isoform A or C or the BORIS gene belonging to the subfamily 5 or 6, wherein the PBL is obtained from the patient.
6. A method of treating a lung cancer or a female reproductive organ cancer comprising intravenously or subcutaneously administering to a subject with the lung cancer or the female reproductive organ cancer:
(a) a polypeptide comprising the amino acids of SEQ ID NO: 3, SEQ ID NO: 10, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67 or SEQ ID NO: 72 and having HLA-A11 antigen-binding capacity or HLA-A24 antigen-binding capacity, or
(b) a polypeptide comprising the amino acids of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 10, SEQ ID NO: 47, or SEQ ID NO: 57, and having HLA-A2 antigen binding capacity,
wherein the administered polypeptide induces CTL upon contact with PBLs in the subject expressing on the cell surface the HLA-A11 antigen or the HLA-A24 antigen or the polypeptide of (b) with the PBLs expressing on the cell surface the HLA-A2 antigen,
wherein the lung cancer or the cancer in a female specific reproductive organ cancer expresses a BORIS gene belonging to isoform A or C or a BORIS gene belonging to subfamily 5 or 6,
wherein the subject administered the polypeptide of (a) is the HLA-A11 antigen positive or the HLA-A24 antigen positive, or the subject administered the polypeptide of (b) is the HLA-A2 antigen positive, and
wherein the CTL induction treats the lung cancer or the female reproductive organ cancer.
7. The method according to claim 6 , wherein the lung cancer or the female reproductive organ cancer comprises a cancer stem cell.