Prism, prism production method, mold, and sensor chip
A prism ( 1090 ) is configured from a dielectric medium and is used in analysis using surface plasmons. The prism ( 1090 ) is provided with an incidence surface ( 1170 ) on which excitation light from outside is incident, a reflection surface ( 1172 ) on which excitation light having entered the incidence surface ( 1170 ) is reflected, an emission surface ( 1174 ) from which excitation light reflected by the reflection surface ( 1172 ) is emitted, and an opposing surface ( 1175 ) opposing the reflection surface ( 1172 ). A gold film ( 1092 ) is formed on the reflection surface ( 1172 ). The opposing surface ( 1175 ) has a sink-mark surface ( 1200 ), and the sink-mark surface ( 1200 ) is a transparent surface.
1. A prism which is constituted by a dielectric medium and is used for an analysis utilizing surface plasmon, comprising:
an incidence surface through which excitation light enters from an outside;
a reflection surface on which the excitation light which has entered through the incidence surface is reflected;
an emission surface through which the excitation light which has been reflected on the reflection surface exits; and
an opposing surface which is opposed to the reflection surface,
wherein a metal film is formed on the reflection surface,
wherein the opposing surface comprises a sink-mark surface,
wherein the sink-mark surface is transparent,
wherein a surface roughness Ra of the sink-mark surface is from 0.1 nm to less than 0.5 μm, and
wherein the sink-mark surface comprises a curved surface which forms a convexity towards the reflection surface.
2. The prism according to claim 1 ,
wherein the sink-mark surface is disposed vertically right below an area including an antigen trapping position of the metal film.
3. A method for producing the prism according to claim 1 , comprising:
preparing a mold with a certain cavity;
injecting a certain resin to fill the cavity;
applying a dwell pressure to the resin; and
cooling the resin,
wherein the mold comprises a transfer area for forming the sink-mark surface, and
wherein a surface roughness Ra of the transfer area is from 0.1 nm to less than 0.5 μm.
4. A method for producing the prism according to claim 1 , comprising:
preparing a mold with a certain cavity;
injecting a certain resin to fill the cavity;
applying a dwell pressure to the resin; and
cooling the resin,
wherein the opposing surface of the mold comprises a first transfer area for forming the sink-mark surface and a second transfer area for forming a non-sink-mark surface between the sink-mark surface and the incidence surface or the emission surface,
wherein a surface roughness Ra of the first transfer area is from 0.1 nm to less than 0.5 μm, and
wherein a surface roughness Ra of the second transfer area is from 0.5 μm to less than 100 μm.
5. A mold for producing the prism according to claim 1 , comprising:
a transfer area for forming the sink-mark surface,
wherein a surface roughness Ra of the transfer area is from 0.1 nm to less than 0.5 μm.
6. A mold for producing the prism according to claim 1 , comprising:
a first transfer area for forming the sink-mark surface of the opposing surface and a second transfer area for forming a non-sink-mark surface of the opposing surface between the sink-mark surface and the incidence surface or the emission surface,
wherein a surface roughness Ra of the first transfer area is from 0.1 nm to less than 0.5 μm, and
wherein a surface roughness Ra of the second transfer area is from 0.5 μm to less than 100 μm.
7. A sensor chip, comprising:
the prism according to claim 1 ; and
a channel forming body in which a channel is formed.
8. The prism according to claim 1 , wherein the metal film is made of Gold.
9. The prism according to claim 8 , wherein the Gold film has a film thickness of from 40 nm to 50 nm.
10. The prism according to claim 9 , wherein the prism is made of a resin material comprising a cycloolefin polymer.
11. The prism according to claim 10 , wherein water absorption of the prism is equal to or less than 0.2%.
12. The prism according to claim 11 , wherein the resin material of the prism has a photoelastic coefficient equal to or less than 80×10 −12 Pa −1 .