Composition and solution with controlled calcium ion level, and related method and use for reperfusion
A solution comprises a preservation mixture comprising a calcium ion source; and a buffer for maintaining a pH of the solution. The molar concentration of calcium ion (Ca 2+ ) in the solution is from 0.18 to 0.26 mmol/L, and the pH is lower than 7.4 and higher than 6.6. A composition for preparing the solution may comprise adenosine, lidocaine, and a calcium source, wherein the molar ratio of adenosine:calcium is from 0.3:0.26 to 0.45:0.18, and the molar ratio of lidocaine:calcium is from 0.04:0.26 to 0.09:0.18. A donor heart may be reperfused with the solution. The solution may be used for reperfusion of a donor heart, such as at a temperature from about 25 to about 37° C. The donor may be a donor after circulatory death.
1. A solution comprising:
a cardioplegia mixture comprising a calcium ion source and lidocaine; and
a buffer for maintaining a pH of the solution,
wherein the solution comprises 0.04 to 0.09 mmol/L of lidocaine and 0.18 to 0.26 mmol/L of calcium ion (Ca 2+ ), and the pH is lower than 7.4 and higher than 6.6.
2. The solution of claim 1 , wherein the molar concentration of calcium ion (Ca 2+ ) is 0.22 mmol/L.
3. The solution of claim 1 , wherein the pH is from 6.8 to 7.0.
4. The solution of claim 1 , wherein the pH is 6.9.
5. The solution of claim 1 , wherein the cardioplegia mixture further comprises adenosine and a magnesium ion source.
6. The solution of claim 5 , comprising 0.3 to 0.45 mmol/L of adenosine, and 11 to 15 mmol/L of Mg 2+ .
7. The solution of claim 1 , further comprising a sodium ion source and a potassium ion source.
8. The solution of claim 7 , comprising about 130 to about 160 mmol/L of Na + and 4 to 7 mmol/L of K + .
9. The solution of claim 1 , further comprising chloride, an osmotic buffer and a reducing agent.
10. The solution of claim 1 , further comprising 70 to 180 mmol/L chloride, 8 to 12.5 mmol/L of glucose, 7.5 to 12.5 IU/L of insulin, 100 to 140 mmol/L of D-mannitol, 0.75 to 1.25 mmol/L of pyruvate, and 2.5 to 3.5 mmol/L of reduced glutathione.
11. The solution of claim 1 , wherein the solution is oxygenated.
12. The solution of claim 1 , comprising:
0.3 to 0.45 mmol/L of adenosine;
0.04 to 0.09 mmol/L of lidocaine;
8 to 12.5 mmol/L of glucose;
110 to 130 mmol/L of NaCl;
4 to 7 mmol/L of KCl;
16 to 24 mmol/L of NaHCO 3 ;
0.9 to 1.4 mmol/L of NaH 2 PO 4 ;
0.18 to 0.26 mmol/L of CaCl 2 ;
11 to 15 mmol/L of MgCl 2 ;
7.5 to 12.5 IU/L of insulin;
100 to 140 mmol/L of D-mannitol;
0.75 to 1.25 mmol/L of pyruvate; and
2.5 to 3.5 mmol/L of reduced glutathione.
13. The solution of claim 1 , comprising:
0.4 mmol/L of adenosine;
0.05 mmol/L of lidocaine;
10 mmol/L of glucose;
123.8 mmol/L of NaCl;
5.9 mmol/L of KCl;
20 mmol/L of NaHCO 3 ;
1.2 mmol/L of NaH 2 PO 4 ;
0.22 mmol/L of CaCl 2 ;
13 mmol/L of MgCl 2 ;
10 IU/L of insulin;
120 mmol/L of D-mannitol;
1 mmol/L of pyruvate; and
3 mmol/L of reduced glutathione.
14. A method comprising reperfusing a donor heart with the solution of claim 1 .
15. The method of claim 14 , wherein the heart is reperfused with the solution during removal of the heart from a donor of the heart.
16. The method of claim 14 , wherein the heart is reperfused with the solution in a reperfusion device.
17. The method of claim 16 , wherein the heart is reperfused with the solution for at least 3 minutes immediately after removal of the heart from a donor of the heart.
18. The method of claim 14 , wherein the heart is removed from a donor after circulatory death.
19. The method of claim 14 , wherein the heart is at a temperature above about 25° C. and below about 37° C.