Method for producing radiobacteria for therapy of cancer
View Patent ↗A method of producing radiobacteria is provided, especially radiolisteria- 32 P. Compositions and methods of use thereof are also provided.
1. A method for producing radiobacteria comprising obtaining a culture of attenuated Listeria monocytogenes bacteria, starving said bacteria for 5 to 60 minutes in a saline-based phosphate-free solution, and subsequently growing the bacteria in is a radionuclide-containing medium supplemented with 32 P which is otherwise phosphate-free for 50-70 minutes so as to produce radiobacteria.
2. The method of claim 1 , wherein the bacteria are grown for 60 minutes in the radionuclide-containing medium.
3. The method of claim 1 , further comprising subsequently washing the radiobacteria and recovering them.
4. A method for treating a tumor in a subject, or for reducing or preventing metastasis of a tumor in a subject, comprising administering to the subject an amount of radiobacteria produced by the method of claim 1 effective to treat the tumor in the subject, or so as to reduce or prevent metastasis of the tumor in the subject.
5. The method claim 4 , wherein the bacteria from which the radiobacteria are produced are Listeria monocytogenes.
6. The method of claim 4 , wherein the radiobacteria are administered systemically to the subject.
7. The method of claim 4 , wherein the radiobacteria are administered locally to the tumor in the subject.
8. The method of claim 7 , wherein the radiobacteria are injected into the tumor in the subject.
9. The method of claim 4 , wherein the tumor is a pancreatic tumor.
10. The method of claim 4 , wherein the tumor is a tumor of the ovary, uterus, neck, head, breast, prostate, liver, lung, kidney, neurones, glia, colon, testicle, or bladder or is a hepatocellular cancer.
11. The method of claim 4 , wherein the tumor is an inoperable tumor.
12. The method of claim 4 , wherein the amount of radiobacteria administered provides a radiation dose of 1-500 mCi.
13. The method of claim 4 , wherein the amount of radiobacteria administered provides a radiation dose of 1-5 mCi.
14. The method of claim 4 , wherein the method is for treating a tumor in a subject.
15. The method of claim 4 , wherein the method is for reducing or preventing metastasis of a tumor in a subject.