Modified kappa light chain-binding polypeptides
View Patent ↗A kappa light chain-binding polypeptide comprising or consisting essentially of one or more binding domains of Peptostreptococcus Protein L, each of said domains being selected from the group consisting of domain 2, domain 3 and domain 4.
1. A polypeptide comprising the amino acid sequence selected from the group consisting of sequences defined by SEQ ID NOS: 15-18.
2. The polypeptide of claim 1 , further comprising at the C-terminus or N-terminus one or more coupling elements, selected from the group consisting of a cysteine residue, a plurality of lysine residues and a plurality of histidine residues.
3. The polypeptide of claim 1 , further comprising an N-terminal moiety.
4. The polypeptide of claim 1 , further comprising a C-terminal moiety.
5. A nucleic acid or a vector encoding a polypeptide according to claim 1 .
6. An expression system, which comprises a nucleic acid or vector according to claim 5 .
7. A separation matrix, wherein a plurality of polypeptides or multimers of claim 1 have been coupled to a solid support.
8. The separation matrix according to claim 7 , wherein the polypeptides or multimers have been coupled to the solid support by multipoint attachment.
9. The separation matrix according to claim 7 , wherein the solid support is a polysaccharide.
10. The polypeptide of claim 1 , comprising the amino acid sequence defined by SEQ ID NO:15.
11. The polypeptide of claim 1 , comprising the amino acid sequence defined by SEQ ID NO:16.
12. The polypeptide of claim 1 , comprising the amino acid sequence defined by SEQ ID NO:17.
13. The polypeptide of claim 1 , comprising the amino acid sequence defined by SEQ ID NO:18.