Cosmetic composition for improving condition of skin, containing cell culture medium, epidermal growth factor and bovine serum albumin
The present invention relates to: a cosmetic composition for improving the condition of the skin, containing, as active ingredients, a cell culture medium, an epidermal growth factor and a bovine serum albumin; a method for improving the condition of the skin by using the same; and a method for preventing or treating skin diseases. According to the present invention, the cosmetic composition can exhibit excellent wrinkle-reducing and wound-healing effects even when only a small amount of EGF is used, and thus can be useful in improving the condition of the skin or in preventing or treating skin diseases.
1. A method for improving a skin condition of a subject in need thereof, comprising applying to skin of the subject a composition comprising:
a) a cell culture medium;
b) 0.4 to 0.8 ng/mL of epidermal growth factor (EGF); and
c) 10 to 100 μg/mL of bovine serum albumin (BSA),
wherein the a) cell culture medium is a fetal bovine serum-free medium,
wherein the improvement of a skin condition is selected from the group consisting of inhibition of wrinkle occurrence, inhibition of skin aging, improvement of skin elasticity, skin regeneration, injury or wound healing, and a combination thereof; and
wherein the subject in need thereof is a mammal.
2. The method of claim 1 , wherein the cell culture medium is a mammalian cell culture medium.
3. The method of claim 2 , wherein the mammalian cell culture medium is selected from the group consisting of DMEM/F-12 (Dulbecco's Modified Eagle's Medium/Nutrient Mixture F-12), DMEM, and MEM α (Minimum Essential Medium α).
4. The method of claim 1 , wherein the composition further comprises a component selected from the group consisting of an amino acid, a vitamin, a carbon source, an inorganic salt, and a combination thereof.
5. The method of claim 4 , wherein the amino acid is selected from the group consisting of glycine, L-alanine, L-arginine hydrochloride, L-asparagine-monohydrate, L-aspartic acid, L-cysteine hydrochloride-monohydrate, L-cysteine 2HCl, L-glutamic acid, L-glutamine, L-histidine hydrochloride-monohydrate, L-isoleucine, L-leucine, L-lysine hydrochloride, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine disodium dihydrate, L-valine, L-histidine, L-tyrosine disodium salt, and a mixture thereof.
6. The method of claim 4 , wherein the vitamin is selected from the group consisting of biotin, calcium D-pantothenate, folic acid, niacinamide, pyridoxine hydrochloride, riboflavin, thiamine hydrochloride, vitamin B12, choline chloride, i-inositol, ascorbic acid and a mixture thereof.
7. The method of claim 4 , wherein the carbon source is selected from the group consisting of D-glucose (dextrose), sodium pyruvate, hyphoxanthin Na, thymidine, linoleic acid, lipoic acid, adenosine, cytidine, guanosine, uridine, 2′-deoxyadenosine, 2′-deoxycytidine HCl, 2′-deoxyguanosine, and a mixture thereof.
8. The method of claim 4 , wherein the inorganic salt is selected from the group consisting of anhydrous calcium chloride (CaCl 2 ), copper sulfate pentahydrate (CuSO 4 -5H 2 O), ferric sulfate heptahydrate (FeSO 4 -7H 2 O), anhydrous magnesium chloride, anhydrous magnesium sulfate (MgSO 4 ), potassium chloride (KCl), sodium chloride (NaCl), anhydrous disodium hydrogen phosphate (Na 2 HPO 4 ), sodium hydrogen phosphate monohydrate (NaH 2 PO 4 -H 2 O), zinc sulfate heptahydrate (ZnSO 4 -7H 2 O), ferric nitrate nonahydrate (Fe(NO 3 ) 3 ·9H 2 O), sodium bicarbonate (NaHCO 3 ), and a mixture thereof.
9. The method of claim 4 , wherein the composition comprises 1.110 to 1.606 g/L of an amino acid, 0.027 to 0.060 g/L of a vitamin, 1.201 to 4.625 g/L of a carbon source and 9.838 to 10.923 g/L of an inorganic salt.
10. The method of claim 1 , wherein the mammal is human.