Patent
Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from Brassica napus.
1. A gene expression cassette comprising a promoter operably linked to a heterologous coding sequence, wherein the promoter comprises a polynucleotide that shares a sequence identity of at least 98% to SEQ ID NO:1.
2. The gene expression cassette of claim 1 , wherein the polynucleotide comprises SEQ ID NO: 1.
3. The gene expression cassette of claim 1 , wherein the polynucleotide comprises an intron.
4. The gene expression cassette of claim 1 , wherein the polynucleotide comprises a 5′-untranslated region.
5. A recombinant vector comprising the gene expression cassette of claim 1 .
6. A transgenic cell transformed with the gene expression cassette of claim 1 .
7. A transgenic plant comprising the transgenic cell of claim 6 .
8. The transgenic plant of claim 7 , wherein the plant is selected from the group consisting of an Arabidopsis plant, a tobacco plant, a soybean plant, a canola plant and a cotton plant.
9. A transgenic seed comprising the gene expression cassette of claim 1 .
10. The gene expression cassette of claim 1 , wherein the promoter consists of SEQ ID NO:1.
11. A transgenic cell transformed with a synthetic polynucleotide that shares a sequence identity of at least 98% to SEQ ID NO:1.
12. The transgenic cell of claim 11 , wherein the synthetic polynucleotide comprises SEQ ID NO:1.
13. The transgenic cell of claim 11 , wherein the synthetic polynucleotide comprises a 5′-untranslated region.
14. The transgenic cell of claim 11 , wherein the transgenic cell is a transgenic plant cell.
15. A transgenic plant comprising the transgenic plant cell of claim 14 .
16. The transgenic plant of claim 15 , wherein the plant is selected from the group consisting of an Arabidopsis plant, a tobacco plant, a soybean plant, a canola plant and a cotton plant.
17. A transgenic seed comprising the transgenic cell of claim 11 .
18. The transgenic cell of claim 11 , wherein the synthetic polynucleotide consists of SEQ ID NO: 1.
19. A gene expression cassette comprising a polynucleotide sharing at least 98% sequence identity to SEQ ID NO: 1 operably linked to a heterologous coding sequence and to a 3′-untranslated region.
20. A recombinant vector comprising the gene expression cassette of claim 19 .
21. A transgenic cell transformed with the gene expression cassette of claim 19 .
22. The transgenic cell of claim 21 , wherein the transgenic cell is a transgenic plant cell.
23. A transgenic plant comprising the transgenic plant cell of claim 22 .
24. The transgenic plant of claim 23 , wherein the plant is selected from the group consisting of an Arabidopsis plant, a tobacco plant, a soybean plant, a canola plant and a cotton plant.
25. A transgenic seed from the transgenic plant of claim 23 .
26. The gene expression cassette of claim 19 , wherein the polynucleotide sequence consists of SEQ ID NO: 1.