Long-term cell-cultivation using polyimide porous membrane and cell-cryopreservation method using polyimide porous membrane

1. A long term cell culturing method including:

(1) applying cells to a porous polyimide film, and

(2) applying the porous polyimide film to which the cells have been applied, to a cell culture medium and culturing the cells for 30 days or longer without subculturing procedures;

wherein the porous polyimide film has a multilayer structure, having at least two surface layers (an A-surface and a B-surface), and a macro-void layer sandwiched between the two surface layers,

a mean pore size of holes in the A-surface is smaller than a mean pore size of holes in the B-surface, and

the macro-void layer has a partition bonded to the surface layers (the A-surface and the B-surface), and a plurality of macro-voids surrounded by the partition and the surface layers (the A-surface and the B-surface), and

wherein the porous polyimide film is not pre-treated by a cell adhesive substance.

2. The method according to claim 1 , wherein the cells are cultured for 60 days or longer without subculturing procedures in step (2).

3. The method according to claim 1 , wherein the cells are cultured for 120 days or longer without subculturing procedures in step (2).

4. The method according to claim 1 , using two or more porous polyimide films layered either above and below or left and right in the cell culture medium.

5. The method according to claim 1 , wherein the polyimide film is, or two or more porous polyimide films are:

i) folded,

ii) wound into a roll,

iii) connected as sheets or fragments by a filamentous structure, or

iv) bound into a rope,

and used by suspension or anchoring in the cell culture medium in the cell culturing vessel.

6. The method according to claim 1 , wherein in the culturing of step (2), the porous polyimide film or a portion thereof is not in contact with the liquid phase of the cell culture medium.

7. The method according to claim 1 , wherein in the culturing of step (2), the total volume of the cell culture medium in the cell culturing vessel is 10,000 times or less of the total sum of the porous polyimide film volume including the cell survival zone.

8. The method according to claim 1 , wherein in the culturing of step (2), the total volume of the cell culture medium in the cell culturing vessel is 100 times or less of the total sum of the porous polyimide film volume including the cell survival zone.

9. The method according to claim 1 , wherein the culturing in step (2) is carried out in a system in which a cell culture medium is continuously or intermittently supplied to a cell culturing vessel from cell culture medium supply means installed outside of the cell culturing vessel.

10. The method according to claim 9 , wherein the cell culture medium is circulated between the cell culture medium supply means and the cell culturing vessel.

11. The method according to claim 9 , wherein the system is a cell culturing apparatus including a culturing unit which is the cell culturing vessel, and a culture medium-supply unit which is the cell culture medium supply means, wherein

the culturing unit is a culturing unit that houses one or more porous polyimide films to support cells, and that comprises a culture medium supply port and a culture medium discharge port, and

the culture medium-supply unit is a culture medium-supply unit comprising a culture medium housing vessel, a culture medium supply line, and a liquid conveyance pump that conveys the medium continuously or intermittently through the culture medium supply line, the first end of the culture medium supply line contacting the medium in the culture medium housing vessel, and the second end of the culture medium supply line being in communication with the culturing unit interior via the culture medium supply port of the culturing unit.

12. The method according to claim 11 , wherein the culturing unit further comprises a culture medium discharge line, the first end of the culture medium discharge line being connected to the culture medium housing vessel, the second end of the culture medium discharge line being in communication with the culturing unit interior via the culture medium discharge port of the culturing unit, and the medium being able to circulate through the culture medium-supply unit and the culturing unit.

13. The method according to claim 12 , wherein the culturing unit is a culturing unit that does not comprise an air supply port, an air discharge port and an oxygen exchange membrane.

14. The method according to claim 1 , wherein the cells are selected from the group consisting of animal cells, insect cells, plant cells, yeast cells and bacteria.

15. The method according to claim 14 , wherein the animal cells are cells derived from an animal belonging to the subphylum Vertebrata.

16. The method according to claim 15 , wherein the cells are selected from the group consisting of CHO cells, CHO-K1 cell lines, CHO DP-12 cell lines, CHO cell-related lines, Vero cells and MDCK cells.

17. The method according to claim 1 , wherein the porous polyimide film includes a polyimide obtained from a tetracarboxylic dianhydride and a diamine.

18. The method according to claim 17 , wherein the porous polyimide film is a colored porous polyimide film obtained by forming a polyamic acid solution composition including a polyamic acid solution obtained from a tetracarboxylic dianhydride and a diamine, and a coloring precursor, and then heat treating it at 250° C. or higher.