Blister assembly

1. A blister assembly comprising:

a first layer having at least one blister chamber;

a second layer sealed to the first layer, at least around a periphery of the at least one blister chamber, such that a liquid is contained between the first layer and the second layer, wherein the seal between the first and second layers comprises a first planar portion having a first peel strength and a second planar portion having a second peel strength, and wherein the second peel strength is greater than the first peel strength, such that when pressure is applied to the blister, the first planar portion of the seal breaks to release the liquid contained within the chamber into a liquid inlet; and

at least one discontinuity comprising a pimple formed in the first and second layers in a region of the second planar portion of the seal, the first and second layers remaining sealed to each other throughout the discontinuity such that a shear force is required to delaminate the first and second layers in said region of the second portion of the seal, causing the second peel strength to be greater than the first peel strength.

2. The blister assembly of claim 1 , comprised in a fluidic cartridge for carrying out a diagnostic test on a sample introduced into the cartridge.

3. The blister assembly of claim 1 , wherein the at least one discontinuity has a substantially circular footprint.

4. The blister assembly of claim 1 , wherein the at least one discontinuity protrudes from the blister assembly in the same direction as the collapsible blister.

5. The blister assembly of claim 1 , wherein the blister assembly defines a first plane P, from which the at least one collapsible blister and the at least one discontinuity project, and wherein the profile of the discontinuity comprises first and second side portions and an end portion connecting the first and second side portions.

6. The blister assembly of claim 5 , wherein at least a portion of the first and/or second side portions of the discontinuity is aligned radially to the blister.

7. The blister assembly of claim 5 , wherein a profile of the at least one discontinuity has a height of between 0.1 mm and 1.5 mm.

8. The blister assembly of claim 6 , wherein at least one of the first and second side portions extend at an angle α from a plane perpendicular to the plane P, wherein α is less than 30 degrees.

9. The blister assembly of claim 8 , wherein α is less than 15 degrees.

10. The blister assembly of claim 8 , wherein α is 0 degrees.

11. The blister assembly of claim 1 , wherein the profile of the at least one discontinuity is approximately hemispherical.

12. The blister assembly of claim 1 , wherein a profile of the at least one discontinuity has a width of between approximately 0.5 mm and 3 mm.

13. The blister assembly of claim 1 , comprising a plurality of discontinuities.

14. The blister assembly of claim 13 , wherein the plurality of discontinuities are arranged as a dotted or dashed line around at least a portion of the permanent seal.

15. The blister assembly of claim 1 , comprising two or more collapsible blisters, wherein the at least one discontinuity is formed between the collapsible blisters.

16. The blister assembly of claim 1 , wherein the first layer and the second layer comprise a plastic coated foil.

17. The blister assembly of claim 1 , wherein at least one of the first portion of the seal and the second portion of the seal is formed by heat sealing.

18. The blister assembly of claim 1 , comprising first, second and third collapsible blisters, and the liquid contained in the first, second and third blisters are liquid reagents necessary for conducting a preferred test on a sample contained within a fluidic cartridge.

19. The blister assembly of claim 18 , wherein the reagents are selected from lysis buffer, wash buffer and elution buffer.