Diagnostic methods, therapeutic agents and uses thereof
The present invention provides a method for diagnosing a disease or disorder selected from the group consisting of insulin resistance, a metabolic disorder, diabetes and pre-diabetes in a subject. The method comprises the step of determining the level of a compound represented by structural formula (VI): or a salt thereof. Compositions and method of making thereof are also described.
1. A method of preparing a compound represented by the following formula:
or a salt thereof, comprising the steps of:
(1) isolating a compound of formula 3 from Ailanthus triphysa
(2) reacting the compound of formula 3 with an oxidizing reagent to give a compound of formula 4:
(3) reacting the compound of formula 4 with a reducing reagent to give a compound of formula 5:
(4) reacting the compound of formula 5 with sulfuric acid to give the compound of formula (VII).
2. The method of claim 1 , wherein the compound of formula 3 is isolated by extracting the resins of Ailanthus triphysa with an organic solvent.
3. The method of claim 2 , wherein the organic solvent is hexane.
4. The method of claim 1 , wherein the oxidizing reagent is PCC.
5. The method of claim 1 , wherein the reducing reagent is LiAlH 4 or NaBH 4 .
6. The method of claim 1 , wherein the compound of formula (VII) is labeled.