Antibody-mediated neutralization of Ebola viruses
The present disclosure is directed to antibodies binding to and neutralizing ebolavirus and methods for use thereof. The present disclosure is directed to a method of detecting an ebolavirus infection in a subject comprising (a) contacting a sample from said subject with an antibody or antibody fragment having clone-paired heavy and light chain CDR sequences from Table 2, or an antibody fragment thereof; and (b) detecting ebolavirus glycoprotein in said sample by binding of said antibody or antibody fragment to antigen in said sample. In still further embodiments, the present disclosure concerns immunodetection kits for use with the immunodetection methods described above.
1. A method of detecting an ebolavirus infection in a subject comprising:
(a) contacting a sample from said subject with an antibody or antibody fragment having heavy chain CDR sequences of SEQ ID NOS: 130, 131 and 132, and light chain CDR sequences of SEQ ID NO: 201, KVS and SEQ ID NO: 202; and
(b) detecting ebolavirus glycoprotein in said sample by selective binding of said antibody or antibody fragment to ebolavirus glycoprotein in said sample.
2. The method of claim 1 , wherein said sample is a body fluid.
3. The method of claim 1 , wherein said sample is blood, sputum, tears, saliva, mucous or serum, semen urine or feces.
4. The method of claim 1 , wherein detection comprises ELISA, RIA, FACS or Western blot.
5. The method of claim 1 , further comprising performing steps (a) and (b) a second time and determining a change in the glycoprotein levels as compared to the first assay.
6. The method of claim 1 , wherein the antibody or antibody fragment comprises a heavy chain variable region comprising SEQ ID NO: 253 and a light chain variable region comprising SEQ ID NO: 254.
7. The method of claim 1 , wherein said antibody or antibody fragment is encoded by heavy chain and light chain variable sequences having 70%, 80%, or 90% identity to SEQ ID NO: 315 and SEQ ID NO: 316, respectively.
8. The method of claim 1 , wherein said antibody or antibody fragment is encoded by heavy and light chain variable sequences having 95% identity to SEQ ID NO: 315 and SEQ ID NO: 316, respectively.
9. The method of claim 1 , wherein said antibody or antibody fragment comprises heavy and light chain variable sequences having 70%, 80%, or 90% identity to SEQ ID NO: 253 and SEQ ID NO: 254, respectively.
10. The method of claim 1 , wherein said antibody or antibody fragment comprises heavy and light chain variable sequences having 95% identity to SEQ ID NO: 253 and SEQ ID NO: 254, respectively.
11. The method of claim 1 , wherein the antibody fragment is a recombinant scFv (single chain fragment variable) antibody, Fab fragment, F(ab′)2 fragment, or Fv fragment.