Cell maintainer for autologous cell therapy production
In some aspects, the invention relates to automated cell culture incubators and their methods of use. In one aspect, the disclosure provides cell culture incubators having an airlock chamber, a storage chamber and/or an internal chamber. In some aspects, the disclosure provides methods for producing autologous mammalian cell cultures.
1. A cell culture incubator comprising:
a transfer chamber;
first and second internal chambers, each having a controlled temperature and gas mixture therein appropriate for the growth of a type of cell for incubation of said type of cell in one or more cell culture vessels in the internal chambers, wherein the first internal chamber comprises a storage location for storing a plurality of cell culture vessels and wherein the second internal chamber comprises at least one imager;
an external door opening from an external environment to the transfer chamber;
a first internal door opening from the transfer chamber to the first internal chamber;
a second internal door opening from the transfer chamber to the second internal chamber, wherein the first internal door and the second internal door each forms a substantially gas-tight seal when closed;
an interlock for preventing the first and second internal doors from opening when the external door is open;
an automated transfer device for moving at least one of the plurality of cell culture vessels between the storage location in the first internal chamber and the at least one imager in the second internal chamber, wherein the transfer device includes at least one robotic element in at least one of the chambers and wherein the storage location is configured to automatically move the at least one of the plurality of cell culture vessels into a position to be transferred by the automated transfer device;
at least one processor configured to control the movement of the at least one of the plurality of cell culture vessels in the storage location and the transfer of the at least one of the plurality of cell culture vessels to and from the storage location; and
radiofrequency sensors for sensing the position of each cell culture vessel in the chambers of the incubator and communicating with the at least one processor to enable the at least one processor to determine the location of each cell culture vessel for desired transfer of each cell culture vessel between the storage location and the at least one imager.
2. The cell culture incubator according to claim 1 , wherein the storage location is configured to receive the at least one of the plurality of cell culture vessels transferred by the automated transfer device and move the at least one of the plurality of cell culture vessels into storage.
3. The cell culture incubator according to claim 2 , wherein the storage location has at least one movable stage to move a cell culture vessel in storage in x-axis, y-axis and z-axis directions to effect transfer to the at least one imager and storage when returned from the at least one imager.
4. The cell culture incubator according to claim 1 , wherein the plurality of cell culture vessels has fiducial marks and wherein the at least one imager uses the fiducial marks to align a cell culture vessel for imaging.
5. The cell culture incubator according to claim 1 , wherein the radiofrequency sensors include radiofrequency beacons in the incubator.
6. The cell culture incubator according to claim 1 , wherein the radiofrequency sensors sense light signals in the incubator.
7. The cell culture incubator according to claim 1 , wherein cell culture vessels include bar codes encoding bar code data and the incubator includes a bar code reader for reading the bar codes and wherein the at least one processor receives the bar code data to enable a user to select a system protocol based at least in part on the bar code data to determine operations to be performed on cells in the corresponding cell culture vessel.
8. The cell culture incubator according to claim 1 , further comprising an ozone generator coupled to a pump, wherein the ozone generator is configured for supplying ozone gas to the transfer chamber.
9. The cell culture incubator according to claim 1 , wherein the external door forms a substantially gas-tight seal when closed.
10. The cell culture incubator according to claim 1 , wherein each cell culture vessel of the plurality of cell culture vessels is tagged with a unique barcode and wherein the incubator further comprises a bar code reader for reading each unique barcode.