Automated cell culture incubator
Aspects relate to an automated cell culture incubator and to methods for using such an incubator. In one aspect, the cell culture incubator includes an incubator cabinet having a transfer chamber and an internal chamber that are arranged to form an airlock configuration. In some embodiments, objects placed into the incubator cabinet are sterilized using ozone produced by an ozone generator. The incubator may include one or more transfer devices that move objects between the transfer chamber and the internal chamber.
1. A cell culture incubator comprising:
an incubator cabinet comprising (i) a transfer chamber and (ii) an internal chamber having a controlled temperature and gas mixture therein appropriate for the growth of a type of cell for incubation of said type of cell, wherein the internal chamber comprises a storage location for storing a plurality of cell culture vessels, and wherein the internal chamber comprises at least one imager;
a sterilization medium source;
a pump operatively coupled with said sterilization medium source and configured for supplying sterilization medium to said transfer chamber; an external door opening from an external environment to said transfer chamber; an internal door opening from said transfer chamber to said internal chamber;
an automated transfer device disposed at least within the internal chamber for moving cell culture vessels through the internal door opening between the storage location in the internal chamber and the at least one imager, wherein the transfer device includes at least one robotic element in at least the internal chamber and wherein the storage location is configured to automatically move at least one of the cell culture vessels into a position to be transferred by the automated transfer device;
a grid of lasers producing light signals located within the transfer and internal chambers of the incubator; and
a controller for receiving the light signals from the grid of lasers to enable the controller to determine the location of each cell culture vessel for the desired transfer of each cell culture vessel between the storage location and the at least one imager.
2. The incubator of claim 1 , wherein said internal chamber is configured to store a plurality of cell culture vessels.
3. The incubator of claim 1 , wherein the sterilization medium source is in fluid communication with said internal chamber.
4. The incubator of claim 1 , wherein said external door forms a substantially gas-tight seal when closed.
5. The incubator of claim 1 , wherein said internal door forms a substantially gas-tight seal when closed.
6. The incubator of claim 1 , wherein said transfer chamber is configured to accept a cell culture vessel.
7. The incubator of claim 1 , wherein the storage location has at least one movable stage to move a cell culture vessel in storage in x-axis, y-axis and z-axis directions to effect transfer to the at least one imager and storage when returned from the at least one imager.
8. The incubator of claim 7 , wherein said controller is located exterior to said incubator cabinet.
9. The incubator of claim 7 , wherein said controller comprises a computer.
10. The incubator of claim 1 , wherein cell culture vessels include bar codes encoding bar code data and the incubator includes a bar code reader for reading the bar codes and wherein the controller receives the bar code data to select a system protocol based at least in part on the bar code data to determine operations to be performed on cells in the corresponding cell culture vessel.
11. The incubator of claim 10 , wherein said cell culture vessel transfer device comprises one or more robotic elements.
12. The incubator of claim 1 , wherein said pump is configured to remove said sterilization medium from said incubator cabinet.
13. The incubator of claim 1 , further comprising an additional pump that is configured to remove said sterilization medium from said incubator cabinet.
14. The incubator of claim 1 , wherein said pump is configured to remove said sterilization medium from said transfer chamber.
15. The incubator of claim 1 , further comprising an additional pump that is configured to remove said sterilization medium from said transfer chamber.
16. The incubator of claim 1 , wherein said transfer device comprises one or more robotic elements.
17. The incubator of claim 1 , wherein said sterilization medium comprises ozone gas.
18. The incubator of claim 1 , wherein said sterilization medium comprises hydrogen peroxide gas.
19. The incubator of claim 1 , wherein said sterilization medium source comprises an ozone generator.
20. The incubator of claim 1 , wherein said sterilization medium source comprises a compressed ozone source.
21. The incubator of claim 1 , wherein said sterilization medium source comprises a compressed hydrogen peroxide source.
22. The incubator of claim 1 , wherein said transfer device comprises one or more robotic elements in the internal chamber.
23. The incubator of claim 1 , wherein said transfer device comprises one or more robotic elements in the internal chamber and in the transfer chamber.
24. The incubator of claim 1 , wherein the light signals are communicated wirelessly to the controller.
25. The incubator of claim 1 , wherein the grid of lasers detect the position of a cell culture vessel without a sensor on the cell culture vessel.