Small molecule metabolites for the diagnosis of bacterial vaginosis and assessment of disease risk
The invention disclosed herein generally relates to methods and kits for diagnosing, assessing disease risk, treating, and preventing bacterial vaginosis (BV) and associated conditions. Additional embodiments include methods for developing metabolic profiles associated with increased disease risk, and developing new approaches to treat BV based on interrupting metabolic networks.
1. A method of diagnosing and treating bacterial vaginosis in a subject, the method comprising:
collecting a sample from the subject;
assessing a small molecule metabolomic profile by measuring levels of indicative metabolites comprising at least two of:
a metabolite associated with the presence of clue cells;
a metabolite associated with the presence of amine odors; and
a metabolite associated with vaginal discharge;
comparing the small molecule metabolomic profile with a diagnostic metabolomic profile;
determining that there is a match between the small molecule metabolomic profile and the diagnostic metabolomic profile of at least 50%, the match being indicative of bacterial vaginosis; and
administering an antibiotic treatment to the subject.
2. The method of claim 1 wherein the sample is vaginal fluid or Cervicovaginal lavage fluid (CVL).
3. The method of claim 1 wherein the method comprises Chromatographic separation and mass spectroscopy.
4. The method of claim 1 wherein the indicative metabolites comprise one or more metabolites selected from the group consisting of lipids, amino acids, nucleotides, co-factors, vitamins, carbohydrates, energy molecules and redox molecules.
5. The method of claim 1 wherein the indicative metabolites comprise:
one or more fatty acids selected from the group consisting of 13-HODE, 12-HETE, Glycerol-3-P, Arachidonate, Carnitine;
one or more nucleotides selected from the group consisting of Oxypurinol, Uracil, Adenosine, AMP, Hypoxanthine, and Urate; or
one or more carbohydrates selected from the group consisting of Glyceraldehyde, N-Acetylneuraminate, PEP, F16BP/F26BP/G16BP, Glucoronate, Glucose, Glutamic acid, Lactate, Oxalic Acid, and Sorbitol.
6. The method of claim 1 wherein the match between the small molecule metabolomic profile and the diagnostic metabolomic profile is at least 80%.
7. A method of monitoring disease progression and treating bacterial vaginosis in a subject, the method comprising
collecting a first sample from the subject at a first time;
assessing a first small molecule metabolomic profile by measuring levels of indicative metabolites comprising at least two of:
a metabolite associated with the presence of clue cells;
a metabolite associated with the presence of amine odors; and
a metabolite associated with vaginal discharge;
comparing the first small molecule metabolomic profile with a diagnostic metabolomic profile;
determining that there is a first match between the first small molecule metabolomic profile and the diagnostic metabolomic profile of at least 50%, the first match being indicative of bacterial vaginosis;
collecting a second sample from the subject at a second time that is after the first time;
assessing a second small molecule metabolomic profile by measuring levels of the indicative metabolites;
comparing the second small molecule metabolomic profile with the diagnostic metabolomic profile;
determining that there is a second match between the second small molecule metabolomic profile and the diagnostic metabolomic profile of at least 50%;
determining disease progression of the bacterial vaginosis based at least in part on the first and second matches; and
administering an antibiotic treatment to the subject.
8. The method of claim 7 wherein the period of time between the first time and the second time is at least three days.
9. The method of claim 7 wherein the first sample, the second sample, or both are vaginal fluid or Cervicovaginal lavage fluid (CVL).
10. The method of claim 1 , wherein the metabolite associate with the presence of clue cells is Deoxycarnitine or Pipecolate.
11. The method of claim 1 , wherein the metabolite associated with the presence of amine odors is N-acetylputrescine.
12. The method of claim 1 , wherein the metabolite associated with vaginal discharge is Agmatine or Cadaverine.
13. The method of claim 1 , wherein the indicative metabolites further comprise Tyramine, Tryptamine, 5-aminovalerate, 4-hydroxybutyrate, or a combination thereof.
14. The method of claim 1 , wherein:
the metabolite associate with the presence of clue cells is Deoxycarnitine or Pipecolate;
the metabolite associated with the presence of amine odors is N-acetylputrescine; and
the metabolite associated with vaginal discharge is Agmatine or Cadaverine.
15. The method of claim 7 , wherein the metabolite associate with the presence of clue cells is Deoxycarnitine or Pipecolate.
16. The method of claim 7 , wherein the metabolite associated with the presence of amine odors is N-acetylputrescine.
17. The method of claim 7 , wherein the metabolite associated with vaginal discharge is Agmatine or Cadaverine.
18. The method of claim 7 , wherein the indicative metabolites further comprise Tyramine, Tryptamine, 5-aminovalerate, 4-hydroxybutyrate, or a combination thereof.