Vector systems for nuclease-mediated genome editing
The invention relates to the field of genetic engineering tools, methods and techniques for gene or genome editing. Specifically, the invention concerns isolated polypeptides having nuclease activity, host cells and expression vectors comprising nucleic acids encoding said polypeptides as well as methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, host cells and methods of the present invention have application in many fields of biotechnology, including, for example, synthetic biology and gene therapy.
1. A vector system comprising one or more vectors, wherein the one or more vectors encode:
a) a Cpf1 polypeptide that has at least 95% sequence identity with the polypeptide of SEQ ID NO: 1, and comprises the amino acid sequence YLFQIYNKDF corresponding to amino acid residues 784-793 of SEQ ID NO: 1; wherein the Cpf1 polypeptide comprises a RuvC domain, does not comprise an HNH domain, and has nuclease activity; and
b) a guide RNA for which said Cpf1 polypeptide has affinity, wherein the guide RNA comprises a sequence substantially complementary to a target nucleic acid sequence.
2. The vector system of claim 1 , wherein the Cpf1 polypeptide comprises the amino acid sequence GKLYLFQIYNKDFS corresponding to amino acid residues 781-794 of SEQ ID NO:1.
3. The vector system of claim 1 , wherein said one or more vectors are viral vectors.
4. The vector system of claim 3 , wherein said viral vectors are Adeno-associated virus (AAV) vectors.
5. A host cell transformed with the vector system of claim 1 .
6. The vector system of claim 1 , wherein the Cpf1 polypeptide is fused at its N or C terminus to an additional protein domain.
7. The vector system of claim 6 , wherein the additional protein domain is a helicase, a nuclease, a nuclease-helicase, a DNA methyltransferase, a DNA demethylase, a histone methyltransferase, a histone demethylase, an acetylase, a deacetylase, a phosphatase, a kinase, a transcription (co-)activator, an RNA polymerase subunit, a transcription repressor, a DNA binding protein, a DNA structuring protein, a marker protein, a reporter protein, a fluorescent protein, a ligand binding protein, a signal peptide, a subcellular localization signal, or an antibody epitope.
8. The vector system of claim 6 , wherein the additional protein domain is a nuclear localization signal.
9. The vector system of claim 6 , wherein the additional protein domain is a FokI domain.