Compositions and methods for administering antibodies
The present invention relates to a drug delivery system and uses thereof. Specifically, a system that can be used to deliver therapeutic proteins, including antibodies, to proteolytic environments is disclosed. In one form of the invention the drug delivery system is a composition which comprises a porous substrate and an antibody bound to the substrate. In one embodiment, the composition comprises nanoporous silicon and can be used to deliver antibodies for the treatment, or for improving the repair, of a wound.
1. A method of treating a wound, the method comprising:
administering a composition to the wound, wherein the composition comprises: a porous silicon substrate, wherein the porous silicon substrate has been thermally oxidized at a temperature less than 600° C.; and a therapeutically effective amount of a wound healing antibody passively bound to pores of the substrate, or passively bound to the surface of the substrate and to pores of the substrate, and
releasing the wound healing antibody from the substrate so as to treat the wound.
2. The method of claim 1 , wherein the porous silicon substrate comprises nanoporous silicon, or mesoporous silicon, or nanoporous silicon and mesoporous silicon.
3. The method of claim 1 , wherein the porous silicon substrate comprises a porosified silicon film produced from a crystalline silicon wafer by more than one etching step.
4. The method of claim 1 , wherein the porous silicon substrate comprises mesoporous nanoparticles, or mesoporous microparticles, or mesoporous nanoparticles and mesoporous microparticles.
5. The method of claim 4 , wherein the mesoporous nanoparticles or mesoporous microparticles are produced by sonication of the porous silicon substrate.
6. The method of claim 4 , wherein the mesoporous nanoparticles comprise an average size of between about 100 nm to about 1000 nm, and the mesoporous microparticles comprise an average size of between about 1 μm to about 500 μm.
7. The method of claim 1 , wherein the porous silicon substrate comprises an average pore size of between about 10 nm to about 40 nm.
8. The method of claim 1 , wherein the wound is an acute wound, a chronic wound, or a wound in an individual with compromised wound healing capacity.
9. The method of claim 8 , wherein the acute wound is the result of a penetrative injury, a burn, nerve damage or from elective surgery, or wherein the chronic wound is a diabetic, veneous, arterial, or decubitus ulcer.
10. The method of claim 1 , wherein the wound healing antibody is a monoclonal antibody to Flightless I, or a monoclonal antibody to TNF-α.
11. The method of claim 1 , wherein the administering comprises: (i) exposing the wound to a dressing or bandage that comprises the composition; or (ii) topical administration of the composition to the wound; or (iii) systemic administration of the composition.
12. A wound healing composition comprising: a porous silicon substrate, wherein the porous silicon substrate has been thermally oxidized at a temperature less than 600° C.; and a therapeutically effective amount of a wound healing antibody passively bound to pores of the substrate, or passively bound to the surface of the substrate and to pores of the substrate, wherein the composition protects the wound healing antibody from degradation in a wound.
13. The composition of claim 12 , wherein the porous silicon substrate comprises nanoporous silicon, or mesoporous silicon, or nanoporous silicon and mesoporous silicon.
14. The composition of claim 12 , wherein the porous silicon substrate comprises mesoporous nanoparticles, or mesoporous microparticles, or mesoporous nanoparticles and mesoporous microparticles.
15. The composition of claim 14 , wherein the mesoporous nanoparticles comprise an average size of between about 100 nm to about 1000 nm, and the mesoporous microparticles comprise an average size of between about 1 μm to about 500 μm.
16. The composition of claim 12 , wherein the porous silicon substrate comprises an average pore size of between about 10 nm to about 40 nm.
17. The composition of claim 12 , wherein the composition is part of a dressing or bandage.
18. The method of claim 10 , wherein the monoclonal antibody to TNF-α is Infliximab.
19. The composition of claim 12 , wherein the wound healing antibody is a monoclonal antibody to Flightless I, or a monoclonal antibody to TNF-α.
20. The composition of claim 19 , wherein the monoclonal antibody to TNF-α is Infliximab.
21. The method of claim 1 , wherein the porous silicon substrate has been thermally oxidized at a temperature less than about 500° C.
22. The method of claim 1 , wherein the porous silicon substrate has been thermally oxidized at a temperature of less than about 400° C.