Prime-boost regimens involving administration of at least one mRNA construct
The present invention relates to prime-boost regimens that involve the administration of at least one mRNA construct, such as the use of such constructs in “boost” administration subsequently to “prime” administration of certain other antigenic composition(s). Such inventive regimens may, in particular, be useful for the induction of an immune response in a subject, and/or the vaccination of such subject against infection from one or more pathogens, and/or the treatment or prevention of one or more diseases or conditions, including a tumour or cancer, allergy or autoimmune conditions, and/or a disease or condition associated with infection from a pathogen. The present invention further describes methods, uses, vaccination compositions, kits and packaged vaccine components related to or useful for one or more of such regimens.
1. A method for inducing an antigen-specific immune response in a subject, the method comprising the steps:
(a) administering to a subject in need thereof at least once an effective amount of a first antigenic composition comprising at least one immunogenic polypeptide at least 100 amino acids in length from a tumor antigen or a pathogen antigen; and
(b) subsequently administering to the subject at least once an effective amount of a second antigenic composition comprising at least one purified mRNA construct that encodes at least one immunogenic polypeptide from the tumor antigen or the pathogen antigen, wherein the at least one immunogenic polypeptide is at least 90% identical to the at least one immunogenic polypeptide comprised in the first antigenic composition,
wherein neither the first antigenic composition nor the second antigenic composition comprises a self-replicating RNA.
2. The method of claim 1 , wherein the at least one immunogenic polypeptide is from a tumor antigen.
3. The method of claim 1 , wherein the at least one immunogenic polypeptide is from a pathogen antigen.
4. The method of claim 3 , wherein the pathogen antigen is a viral antigen.
5. The method of claim 4 , wherein the viral antigen is an influenza hemagglutinin (HA) antigen, an influenza neuraminidase (NA) antigen, a rabies virus glycoprotein (G), a respiratory syncytial virus fusion protein (F) or a coronavirus spike protein (S).
6. The method of claim 1 , wherein the purified mRNA is complexed with cationic lipids thereby forms liposomes, lipid nanoparticles and/or lipoplexes.
7. The method of claim 1 , wherein the first antigenic composition and the second antigenic composition are administered to the subject in need thereof, respectively, in a prime-boost immunisation regimen.
8. The method of claim 1 , wherein the second antigenic composition is subsequently administered within about 28 days of administration of the first antigenic composition.
9. The method of claim 1 , wherein:
the first antigenic composition is administered in two or more doses prior to the administration of the second antigenic composition, or
the second antigenic composition is administered in two or more doses subsequently to the administration of the first antigenic composition.
10. The method of claim 9 , wherein the interval between the administration of one or more pairs of consecutive doses is from about 5 to 120 days.
11. The method of claim 1 , wherein the first antigenic composition or the second antigenic composition is administered by subcutaneous, intramuscular, intradermal, or jet injection.
12. The method of claim 1 , wherein the G/C content of the region of the mRNA construct encoding at least one epitope of the immunogenic peptide or polypeptide is increased compared with the G/C content of the region of the wild type or reference mRNA that encodes the epitope of the immunogenic peptide or polypeptide.
13. The method of claim 1 , wherein the mRNA construct comprises additionally:
(a) a 5′-CAP structure;
(b) a poly(A) sequence; and
(c) optionally a poly (C) sequence.
14. The method of claim 13 , wherein the poly(A) sequence comprises a sequence of about 25 to about 400 adenosine nucleotides.
15. The method of claim 1 , wherein the mRNA construct comprises additionally at least one histone stem-loop comprising a corresponding RNA sequence to the nucleic acid sequence according to SEQ ID NO. 1.
16. The method of claim 1 , wherein the mRNA construct comprises additionally a 3′-UTR element.
17. The method of claim 16 , wherein the 3′-UTR element comprises a nucleic acid sequence derived from a 3′-UTR of a gene selected from the group consisting of an albumin gene, an alpha-globin gene, a beta-globin gene, a tyrosine hydroxylase gene, a lipoxygenase gene, and a collagen alpha gene.
18. The method of claim 1 , wherein the mRNA construct comprises additionally a 5′-UTR element which comprises a nucleic acid sequence which is derived from the 5′-UTR of a TOP gene, or from a corresponding RNA sequence.
19. The method of claim 18 , wherein the 5′-UTR element comprises a nucleic acid sequence which is derived from a 5′-UTR of a TOP gene encoding a ribosomal protein.
20. The method of claim 18 , wherein the mRNA construct comprises:
(a) a 5′-CAP structure;
(b) a 5′-UTR element which comprises a nucleic acid sequence which is derived from the 5′-UTR of a TOP gene;
(c) a coding region encoding the at least one immunogenic peptide or polypeptide;
(d) a 3′-UTR element comprising a nucleic acid sequence which is derived from a gene providing a stable mRNA;
(e) a poly(A) sequence;
(f) a poly(C) sequence; and
(g) a histone-stem-loop.
21. The method of claim 1 , wherein the mRNA construct is associated with or complexed with a cationic or polycationic compound or a polymeric carrier.
22. A packaged immunogenic composition comprising:
a first antigenic composition comprising at least one immunogenic polypeptide at least 100 amino acids in length from a tumor antigen or a pathogen antigen, and
a second antigenic composition comprising at least one purified mRNA construct that encodes at least one immunogenic polypeptide from the tumor antigen or the pathogen antigen, wherein the at least one immunogenic polypeptide encoded by the mRNA construct is at least 90% identical to the at least one immunogenic polypeptide comprised in the first antigenic composition, and
the package comprising additionally instructions to:
(a) administer to a subject in need thereof at least once an effective amount of the first antigenic composition; and
(b) subsequently administer to the subject at least once an effective amount of the second antigenic composition.